Collagen Research

Keywords

Crucian Carp Skin, Collagen Peptide, Calcium Absorption, Low Calcium Dietary Model, Bone Microstructure

Abstract

Objective: To study the calcium absorption-promoting effect of collagen peptides (CPs) in mice. Methods: The mouse low-calcium dietary model method was adopted, Kunming male mice were used as experimental animals, and a normal group, a low-calcium model group, a calcium carbonate group, a low-dose CPs (500 mg/kg mb+CaCO3), and a high-dose (1 000 mg /kg mb£«CaCO3) group and casein phosphopeptide control group were administered orally continuously for 6 weeks to examine the serum calcium, phosphorus, alkaline phosphatase (ALP) activity, calcium absorption rate, various bone indicators and bone microscopic Structural and trabecular bone morphological changes. Results: The ALP activity of mice in CPs low- and high-dose groups was significantly lower than that of the low-calcium model group (P<0.05). The bone length, dry mass index, bone calcium content, bone mineral density (BMD), bone The mineral content (bone mineral content, BMC) was significantly higher than that of the low-calcium model group (P<0.05). The CPs low-dose group could make the various indicators of mice fed low-calcium feed reach the level of the normal group of mice. The ALP activity of mice in the CPs low-dose group was significantly lower than that of the calcium carbonate group (P<0.05), and their bone calcium content, BMD, and BMC were all significantly higher than those of the calcium carbonate group (P<0.05), suggesting that CPs should be supplemented while supplementing calcium carbonate. , which has a better calcium absorption effect. Conclusion: CPs can increase the calcium absorption rate in mice, increase the number and strength of bone trabeculae, and promote bone growth, thereby effectively promoting calcium absorption. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by He Hui, Hou Tao, Liu Weiwei, Liu Yanshuang, Shi Wen, Guo Danjun  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Antioxidant Peptides, Dace, Collagen, Dpph, Orac, Dna Oxidative Damage

Abstract

In order to enzymatically digest low-value protein resources with bacterial extracellular proteases to prepare antioxidant active peptides and discover new proteases, a liquid fermentation culture method was used to ferment Pseudoalteromonas sp. SHK1-2 to produce enzymes and obtain crude extracellular proteases. The enzyme solution is used to enzymatically hydrolyze dace collagen extracted by hot water method. Through ultrafiltration and sephadex LH-20 molecular sieve chromatography, it is obtained with DPPH free radical scavenging ability (35.6% ¡À 7%) and oxygen free radical scavenging ability (Oxygen radical). absorbance capacity (ORAC) and DNA oxidative damage inhibitory activity. Liquid chromatography-mass spectrometry identified the molecular weight of this active peptide as 776.2 Da, and the predicted amino acid sequence is Thr-Ala-Gly-His-Pro-Gly-Thr-His . ORAC testing has verified that synthetic peptides also have antioxidant activity. Research shows that bacterial extracellular proteases have certain application prospects in increasing the value of low-value resources, and it has certain reference significance for the exploration of new proteases and their applications.

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Original research was done by He Hailun, Liu Dan, Wu Ribang, Zhang Jiang, Yang Xinghao, Wu Cuiling, Huang Jiafeng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Scaffold, Collagen Binding Domain, Brain-Derived Neurotrophic Factor, Spinal Cord Injury, Motor Function, Rat

Abstract

Objective To evaluate the effect of collagen scaffold combined with brain-derived neurotrophic factor (BDNF) transplantation in repairing total transverse spinal cord injury in rats. Methods 32 adult female SD rats were randomly divided into 4 groups (n=8): Group A was a sham operation group, only the T9 and T10 spinal cord segments were exposed; Groups B, C, and D had 4 mm long T9 and T10 spinal cord segments removed. Afterwards, groups C and D were implanted with corresponding lengths of linear ordered collagen scaffolds (LOCS) and LOCS combined with collagen binding domain (CBD)-BDNF at the injury site. BBB motor function scores were performed on the rats before surgery and every week within 3 months after surgery. Three months after the operation, the motor evoked potential (MEP) of the rats in each group was detected by neuroelectrophysiology; then fluorogold (FG) was injected into the L2 spinal cord tissue for retrograde tracing, and the tissue was removed 1 week later. The mouse brain and thoracic and lumbar spinal cord tissue were taken. After dehydration, the morphology of the spinal cord tissue was observed; the thoracic and lumbar spinal cord tissue including the injured area was taken for sectioning. Among them, coronal sections of the spinal cord were observed under a laser confocal microscope to calculate the integrated absorbance (IA) value of FG-positive cells; horizontal sections of the thoracic spinal cord tissue were stained with immunofluorescence to observe the modeling of full transverse spinal cord injury and the axons in the spinal cord injury area. Regeneration status, synapse formation status of regenerated axons in group D. Results The BBB scores of groups B, C, and D were significantly lower than those of group A at each time point after operation (P<0.05); the BBB scores of group D were significantly higher than those of groups B and C from 2 to 12 weeks after operation (P<0.05). Electrophysiological testing showed that no MEP was observed in group B; the MEP latency in groups C and D was significantly longer than that in group A, and that in group C was significantly longer than that in group D. The differences were all statistically significant (P<0.05). Observation of spinal cord tissue morphology showed that the spinal cord injury area in group B extended to both ends, and the tissue at the injured site was severely damaged; the spinal cord morphology in groups C and D recovered better, and group D was closer to the normal tissue morphology. Retrograde tracing results showed that the gray matter of the lumbar spinal cord below the injury area of rats in each group was filled with FG-positive cells; in the thoracic spinal cord above the injury area, the IA value in the FG-positive area of group A was significantly greater than that of B, C, and D group (P<0.05), groups C and D were greater than group B (P<0.05), and there was no statistically significant difference between groups C and D (P>0.05). Immunofluorescence staining showed that tissue sections selected from the dorsal to ventral side of the same spinal cord showed a fully transverse spinal cord injury area that was significantly different from normal tissue. The number of NF-positive axons in group A was significantly more than that in groups B, C, and D, that in groups C and D was more than that in group B, and that in group D was more than that in group C. The differences were all statistically significant (P<0.05). Conclusion LOCS combined with CBD-BDNF transplantation can promote axonal regeneration and recovery of hindlimb motor function after total transverse spinal cord injury in rats. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Hou Xianglin, Dai Jianwu, Li Xing, Yang Bin, Xiao Zhifeng, Fan Yongheng, Zhao Yannan, Han Sufang  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Pretreatment Polypeptides Degree Of Hydrolysis Angiotensin-Converting-Enzyme Inhibition Rate

Abstract

This article explores the effects of five pretreatment methods of acid, alkali, heat, ultrasound, and ultrahigh pressure on the preparation of ACE inhibitory peptides from collagen. After pretreatment of collagen, alkaline protease was used to enzymatically hydrolyze the hydrolyzate to prepare a hydrolyzate. The degree of hydrolysis, ACE inhibition rate and molecular weight distribution of the hydrolyzate were measured. The results showed that the degree of hydrolysis and ACE inhibition rate of the hydrolyzate in the alkali and ultrasonic treatment groups were the same. Higher than the untreated group, indicating that these two pretreatment methods may be beneficial to the exposure of collagen triple helix region sites; DSC and infrared analysis of the collagen structure after pretreatment showed that alkali treatment changed the conformational characteristics of collagen and affected non-covalent bonds. The balance of the collagen triple helix region is fully exposed, but the integrity of the subunits is maintained. Ultrasonic treatment destroys the covalent cross-linking of the collagen helix region, exposing more hydrophobic sites, which is conducive to alkaline protease enzymes. However, acid, heat and ultrahigh pressure treatments mainly affect the non-helical regions of collagen and cannot promote the release of collagen ACE inhibitory active peptides during enzymatic hydrolysis. The effects of five pretreatments (acid, alkali, heat, ultrasound, and high pressure) on the production of angiotensin-converting-enzyme (ACE) inhibitory peptide from collagen were explored. After pretreatment, collagen was hydrolyzed by Alcalase, and the degree of hydrolysis (DH), ACE inhibitory activity, and the molecular weight distribution of the hydrolysate were measured. Results showed that alkali- and ultrasound-treated groups had higher DH and ACE inhibition rate than the untreated group, which indicated that these two pretreatments can favor exposure of sites in the triple-helical region of collagen. Differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR) were used to analyze the structure of collagen, and the results showed that alkali treatment changed the configuration of collagen, affected the balance of non-covalent bonds, and exposed the hydrophobic site completely in the triple-helical region of collagen. However, the integrity of the subunits was maintained. Ultrasound treatment damaged the covalent bonds in the helical region of collagen and exposed more hydrophobic sites, which was helpful for alcalase hydrolysis. In contrast, acid, heat, and high-pressure treatments mostly affected the non-helical region of collagen, and could not promote the release of ACE inhibitory peptide from collagen during enzymatic hydrolysis.

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Original research was done by Huang Dandan

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen, Matrix Metalloproteinase, Tissue Inhibitor Of Matrix Metalloproteinase, Inguinal Hernia, Transversalis Fascia

Abstract

¡¾¡¿Objective to compare the expression of collagen, matrix metalloproteinase (mmp)-2, mmp-9 and tissue inhibitor of metalloproteinase (timp)-1, timp-2 in the transversalis fascia and serum of patients with direct inguinal hernia and indirect hernia. To explore the pathogenesis of direct inguinal hernia. Methods The masson method was used to detect the expression of collagen in the transversalis fascia of patients who underwent hernia repair in our hospital (19 cases of direct hernia and 27 cases of indirect hernia). The expression of mmps and timps in the transversalis fascia was compared with the westernblot method. At the same time, elisa was used Method to measure serum mmps and timps. Results: The collagen arrangement of the transversalis fascia in patients with direct hernia was disordered. Compared with patients with direct hernia, the collagen of the transversalis fascia in patients with indirect hernia is more neatly arranged and continuously distributed. The collagen content of the transversalis fascia in patients with direct hernia was significantly lower than that in patients with indirect hernia (p<0.05), and the mmp-2 content in the transversalis fascia and serum was significantly higher than that in patients with indirect hernia ((p<0.05), timp-2 and timp-1 The content was significantly lower than that in patients with indirect hernia (p<0.05). Conclusion? Abnormal changes in mmp-2, timp-2, and timp-1 leading to changes in the extracellular matrix components may be one of the reasons for the occurrence of different types of inguinal hernia. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Li Jian  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Heparin, Collagen, Layer-By-Layer Self-Assembly, Anticoagulant Properties

Abstract

Small-diameter expanded polytetrafluoroethylene (ePTFE) artificial blood vessels are one of the most common alternatives in vascular transplant surgery. However, postoperative thrombosis and restenosis of the vascular lumen are important factors leading to surgical failure. Using an electrostatic layer-by-layer self-assembly method to construct a CD133 functionalized heparin/collagen (HEP/COL) multilayer membrane can inhibit the formation of thrombus and provide the possibility of endothelialization of blood vessels; HEP/COL is tracked through scanning electron microscopy and infrared spectroscopy. The assembly process of the multilayer film, using laser confocal microscopy to demonstrate the grafting of antibodies. The results showed that the CD133 functionalized HEP/COL multilayer membrane successfully covered the surface of the ePTFE artificial blood vessel; the contact angle decreased from 127¡ã to 106¡ã, indicating that the infiltration of the blood vessel was improved; the experimental results of platelet adsorption showed that the modified The number of platelets adsorbed on the surface of the artificial blood vessel is significantly reduced, and it has good anticoagulant properties.

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Original research was done by Zhang Peng, Gong Feirong, Lu Shuyang, Sun Xiaoning, Cheng Shujun

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Membrane, Mitomycin C (Mmc), Non-Penetrating Trabecular Surgery, High Performance Liquid Chromatography

Abstract

Abstract Objective To evaluate the safety of different concentrations of mitomycin C (MMC) collagen membranes used in non-penetrating trabecular surgery. Methods To establish a high-performance liquid chromatography (HPLC) method to determine the vitreous concentration of MMC in rabbit eyes, using centrifugation French-made porcine scleral dry collagen membranes were soaked in MMC solution to make MMC collagen membranes of different concentrations. Healthy purebred Japanese long-eared white rabbits were randomly divided into 6 groups and underwent non-penetrating trabecular surgery on both eyes. A, B, The four groups C and D were jointly implanted with MMC collagen membranes of different concentrations. Group E used 200 mg/L MMC once during the operation, and group F was the blank group. The corneoscleral limbus conditions in the surgical area were compared 1 week after operation. HPLC method was used to detect and compare the MMC concentration in the vitreous cavity of each group at 4 weeks. Results Under this chromatographic condition, the linear range of MMC in the vitreous cavity was 0.1 to 2.5 mg/L, and the correlation coefficient (r) was 0.9970. After surgery At 1 week, the MMC concentration in groups C and D was significantly higher than that in group E (P<0.001); at 4 weeks after surgery, there was no significant difference between group A and group E (P>0.05). Higher than that of group E (P<0.01). Conclusion The concentration of MMC in the vitreous cavity increases with the increase in the concentration of MMC released by the collagen membrane. The concentration of MMC in the vitreous cavity of the collagen membrane loaded with low concentration MMC (50 mg/L) is safer. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Hou Yongsheng, Fu Shaoying, Zhang Xiaoning, Jin Di  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Peptide, Bone Slime, Degreasing, Decalcification, Secondary Orthogonal Rotation Combination Design

Abstract

Bovine bone was prepared into bone mud as the test material, and the conditions for pretreatment degreasing and decalcification for extracting bovine collagen polypeptide were studied. Compare the degreasing effects of three degreasing agents: petroleum ether, absolute ethanol, and n-hexane; use quadratic orthogonal rotation combination design to optimize decalcification conditions based on single factor experiments. The results show that the optimal conditions for the degreasing process are absolute ethanol as the bone mud degreasing agent, bone mud and absolute ethanol material-liquid ratio 1:20 (g/mL), 150r/min vibration soaking for 24 hours, and the bone mud degreasing rate can reach 97.46%. The optimal conditions for the citric acid decalcification process of bone mud are citric acid concentration 0.43 mol/L, decalcification time 16 hours, material-liquid ratio 1:21 (g/mL), and the decalcification rate can reach 96.53%.

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Original research was done by He Guangzhong, Tang Yingying, Luo Aiping, Lang Xiaoqin, Chen Wei

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Rheumatoid Arthritis, Collagen-Induced Arthritis, Ultrasound, Synovitis

Abstract

Objective To establish a stable collagen-induced arthritis (cia) model in rats, try to use high-frequency ultrasound to observe joint lesions in cia rats in vivo, and evaluate the application value of ultrasound in small animal experimental research on RA. Methods 60 wistar rats were divided into model group (50 rats) and blank group (10 rats). In the model group, bovine type II collagen and complete Freund’s adjuvant were intradermally injected into the tail. The immunization was strengthened in the same way 14 days later. The blank group was given normal saline injection at the same time. The joint and extra-articular manifestations of mice in both groups were observed regularly. On the 45th day, ankle joint scores (AI) and high-frequency ultrasound examinations were performed on all experimental rats; the rats were sacrificed, and pathological examinations were performed on a total of 120 ankle joints of 60 rats, and compared with the AI and ultrasound results. As a result, no one in the control group became ill. A total of 100 ankle joints of 50 rats in the model group were pathologically confirmed to have synovitis in 62. Pathological sections showed typical inflammatory cell infiltration, synovial proliferation, cartilage and subchondral bone destruction, and some were accompanied by pannus formation. AI was used to diagnose 42 cases, with a detection rate of 67.8%; high-frequency ultrasound was used to diagnose 54 cases, with a detection rate of 77.4%. There was a significant difference between the two (p<0.05). Conclusion High-frequency ultrasound successfully demonstrated rheumatoid lesions in the ankle joint of cia rats. High-frequency ultrasound can non-invasively reflect joint lesions and can be used as an ideal imaging for studying small animal models of rheumatoid arthritis. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Song Haixia,Wang Quanshi  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Bitongkang Pills, Collagen Arthritis, Tumor Necrosis Factor Alpha

Abstract

Abstract Objective To observe the effect of Bitongkang Pills on serum tumor necrosis factor ¦Á (TNF-¦Á) in rats with collagen-induced arthritis (CIA) and explore its therapeutic mechanism. Methods A rat CIA model induced by type II collagen plus complete Freund’s adjuvant was established. 60 Wistar rats were randomly divided into 6 groups: Bitongkang pill low, medium and high dose groups [0.3 g/(kg¡¤d), 3 g/(kg¡¤d), 30 g/(kg¡¤d)], 3 g/(kg¡¤d) in the Yanbi granule group, 1.2 mg/kg in the methotrexate tablet group every week, and the drug was dissolved in 2 ml of normal saline, once/d, continuously for 4 weeks, and the model group was given 2 ml of normal saline per animal. The rats were sacrificed after 8 weeks, and enzyme-linked immunosorbent assay (ELISA) was used to detect the serum TNF-¦Á concentration of CIA rats, and the changes in TNF-¦Á concentration levels of rats in each group were observed. Results The TNF-¦Á concentration level in the Bitongkang pill group was 1190.84¡À177.02, the TNF-¦Á concentration level in the high-dose group was 844.20¡À142.79, the MTX tablet group was 1149.40¡À182.17, and the Yubi granule group was 1215.83¡À102.07 ) was significantly lower than the model group (1724.67¡À247.26) (P=0.017, P=0.000, P=0.008, P=0.036). The serum TNF-¦Á concentration level in the high-dose Bitongkang Pills group was significantly lower than that in the mid-dose Bitongkang Pills group, MTX tablets group, and Yubi Granules group (P=0.006, P=0.018, P=0.009). Conclusion Bitongkang Pills can effectively reduce the expression of serum TNF-¦Á in CIA rats and inhibit synovial inflammation in CIA rats.

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Original research was done by Lu Xinliang, Jiang Nan, Zhang Jinliang, Xu Mingzhi, Li Huihui

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.