Collagen Research

Keywords

Collagen, Chitosan, Edible Composite Film, Mechanical Properties

Abstract

Edible composite films were prepared using catfish skin collagen and chitosan as raw materials. The influence of process parameters such as the mass ratio of collagen and chitosan, plasticizer type and amount, heat treatment temperature and time on the performance of edible composite films was investigated through orthogonal experiments. The results show that: the mass ratio of collagen to chitosan and the heat treatment temperature have a greater impact on the tensile strength; the addition of glycerin has a significant impact on the elongation at break of the composite film, while the heat treatment time has a small impact on the elongation at break of the composite film. . The preparation process of the composite membrane with the best mechanical properties is as follows: the mass ratio of collagen to chitosan is 6:4, the amount of glycerol added (based on total solute) is 20%, the heat treatment temperature is 70 ¡ãC, and the heat treatment time is 30 min. Under these conditions, the surface of the composite membrane obtained was smooth and bubble-free, the tensile strength was (21.98¡À0.33) MPa, and the elongation at break was (127.35¡À3.03)%.

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Original research was done by Liu Hua, Su Wei, Yuan Meilan, Zhao Li, Chen Lili, Chen Dajia

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Crucian Carp, Hydroxyproline, Collagen, High Performance Liquid Chromatography

Abstract

In order to improve the utilization rate of aquatic product collagen, pre-column derivatization-high performance liquid chromatography was used to detect the hydroxyproline content in crucian carp skin, fish meat, fish scales and swim bladder tissue, and then the hydroxyproline content was converted into Corresponding collagen content. Crucian carp samples were hydrolyzed with hydrochloric acid at 110¡ãC for 18 h, derivatized with dansyl chloride, and detected using high-performance liquid chromatography-ultraviolet method. Chromatographic column: reversed-phase C18 column (250 mm¡Á4.6 mm, 5 ¦Ìm); mobile phase: A is methanol, B is tetrahydrofuran-methanol-0.05 mol/L sodium acetate (1:15:84, V/V) solution, Gradient elution was used; flow rate was 1 mL/min; column temperature was 40¡ãC; detection wavelength was 254 nm. The results show that hydroxyproline has a good relationship in the range of 20 to 400 ¦Ìg/mL (R2=0.999 8), the lowest detection limit is 0.18 ¦Ìg/g, and the average recovery rate at different levels is 80.22% to 83.33%, relatively The standard deviation range is 0.31%~1.84%. Analysis of actual samples showed that the collagen contents in fish skin, fish meat, fish scales, and fish bladder tissues were 106.62, 24.75, 166.94, and 115.05 mg/g respectively. The analysis method established in this experiment is simple, fast, has good sensitivity and reliability, and can be used for the quantitative analysis of collagen in different tissues of aquatic products.

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Original research was done by Liu Fang, Bian Huan, Wu Haihong, Zhang Muhan, Xu Weimin, Wang Daoying, Geng Zhiming, Zhu Yongzhi, Gong Wenyu

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Titanium, Electrostatic Interaction, Type Iv Collagen, Heparin, Endothelial Progenitor Cells

Abstract

Endothelialization on the surface of the scaffold has become a good way to ultimately achieve long-term compatibility between the scaffold and blood and tissue. The accumulation, growth and proliferation of endothelial progenitor cells on the surface of the material make rapid endothelialization possible. This article uses electrostatic binding to combine the anticoagulant drug heparin and type IV collagen, an important component of the endothelial cell basement membrane, to the titanium surface, making it have good cell compatibility. After alkali activation and silanization treatment, titanium forms a positively charged amino surface, which electrostatically combines with the heparin/type IV collagen complex with residual negative charges to form a biochemical layer. The changes of characteristic groups on the surface of the material were detected through Fourier transform infrared grazing, and the changes in surface hydrophobicity and surface morphology were tracked through measurement of water contact angle and scanning electron microscope. Qualitative characterization of type IV collagen by immunofluorescence staining. The results showed that the biomimetic layer was successfully constructed. The modified surface was able to adhere to more endothelial progenitor cells than the titanium surface. The cells grew and multiplied faster, the cell morphology was good, and the cell compatibility was significantly improved. The introduction of type IV collagen has important reference value for the surface modification of cardiovascular implantable devices

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Original research was done by Zhang Kun, Li Jing’an, Wang Xue, Deng Kun, Chen Junying, Huang Nan

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Athletes, Collagen Peptides, Joint Pain, Osteoarthritis

Abstract

Abstract Clinical studies in recent years have proven that collagen peptides can reduce athlete’s joint pain, reduce dependence on analgesics, and enhance exercise ability. Therefore, coloprotein peptide is a safe nutrient supplement for preventing and relieving exercise-induced joint pain.

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Original research was done by Zhou Jianlie, Chen Yue

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Procollagen Gene, Ribozyme, In Vitro Activity, Fibroblasts, Scar

Abstract

Objective To study the cleavage activity and reaction conditions of hammerhead ¦Á1 type I and type III procollagen gene exon 2 fragment ribozymes in vitro on their respective target RNA molecules. The effects of two antisense ribozymes on collagen synthesis by fibroblasts in scars were also observed. Methods The recombinant plasmids (pt-¢¡, pt-¢£) containing exon 2 fragments of ¦Á1 type I and type III procollagen genes were transcribed with 32p label in vitro to form the product target RNA. At the same time, the recombinant plasmids (pt-g¢¡, pt-g¢£) containing specific ribozyme genes were subjected to non-labeled in vitro transcription. The products (ribozymes) were mixed and reacted with their respective 32p-target RNA according to different conditions, and then radiated after electrophoresis. Develop and observe the results. The constructed ribozyme was packaged with liposomes and introduced into cultured fibroblasts, and the effect of the ribozyme on the synthesis of type I and type III collagen mRNA in fibroblasts was observed using image analysis. Results Both ribozymes can effectively cleave their respective target RNAs at 37¡ãC and 42¡ãC, and have a wide range of mg2+ concentration requirements (10~20mmol/l); the reaction temperature gradually dropped from 65¡ãC to 37¡ãC and was maintained at 37¡ãC. Under these conditions, the ribozyme cleavage activity was significantly improved. The expression of type I and III collagen mRNA was significantly reduced, collagen production was reduced, and collagen production was significantly inhibited. Conclusion The ribozyme targeting the exon 2 fragment of ¦Á1 type I and type III procollagen genes can effectively cleave target RNA in vitro and effectively inhibit the synthesis of collagen in fibroblasts in scars.

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Original research was done by Zhu Jiayuan, Zhu Bin, Xu Bing, Zhang Tao, Chen Dong, Tang Bing, Li Xinqiang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Grouper With Oblique Band, Collagen, Composite Membrane, Tea Polyphenols, Preservation

Abstract

Study the preservation effect of collagen-chitosan composite film modified by tea polyphenols on refrigerated grouper. Under refrigerated conditions at 4¡ãC, the samples were processed in three ways: blank control, collagen-chitosan composite film, and tea polyphenol-modified collagen-chitosan composite film and stored for 20 days. Protein content, sulfhydryl (-SH) content, Ca2+-ATPase activity and pH value, thiobarbituric acid (thiobarbityric acid, TBA) value, total volatile basic nitrogen (TVB-N) value, bacterial colonies The total number and sensory evaluation value were used as research indicators. The results show that: the sulfhydryl content, actomyosin content, Ca2+-ATPase activity and sensory evaluation value of grouper treated with collagen-chitosan composite film and tea polyphenol-modified collagen-chitosan composite film. were significantly higher than the control group (P<0.05), while the K value, pH value, TVB-N value, and total bacterial colony count were all significantly lower than the control group (P<0.05), and tea polyphenol modified collagen-chitosan The effect of the composite membrane treatment group was better than that of the collagen-chitosan composite membrane treatment group, and the difference in TBA values among the three treatment groups was not significant (P>0.05). The actomyosin denaturation and sulfhydryl content of fish in the tea polyphenol-modified collagen-chitosan composite membrane treatment group were slow, the decrease in Ca2+-ATPase activity was hindered, the TVB-N value and other physical and chemical indicators were changed, and the growth of total bacterial count was inhibited. . This shows that under 4 ¡æ refrigeration conditions, the tea polyphenol-modified collagen-chitosan composite membrane treatment group can effectively delay the spoilage of grouper, and the preservation effect is more obvious.

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Original research was done by Yu Lin, Wang Qian, Wang Juanjuan, Chen Shunsheng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Collagen Gelatin Gelatinization Microstructurecollagen Gelatin Gelatinization Microstructure

Abstract

This experiment uses pig skin collagen as raw material to study the changes in the microstructure of collagen during the gelatinization process. Research shows that under the induction of 8 hours of acid treatment, the degree of gelatinization of collagen is high, and the gelatin yield after hot water extraction can reach 83.98%. As the acid treatment time is further extended, the gelatin yield decreases significantly; differential calorimetry scanning (DSC), infrared spectroscopy (FTIR) and circular dichroism spectroscopy (CD) studies show that as acid treatment proceeds, the triple helix structure of collagen gradually loosens, and the valence bonds that maintain the stability of the triple helix structure are gradually destroyed. After 8 hours of acid treatment, , the excessive destruction of the collagen triple helix structure is the main reason for the decrease in gelatin yield. Polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that during acid treatment, degradation of collagen peptide chains always existed, and the amount of subunits retained in gelatinized collagen directly affected the subunit content in gelatin. Therefore, a balance must be found between the degradation of the peptide chain and the loosening of the triple helix structure during the gelatinization process to obtain high yield and high-quality gelatin. The microstructure of collagen from pig skin was studied during gelatinization. The results showed that a relatively high degree of gelatinization of collagen was achieved after 8-h acid treatment and the yield of gelatin reached 83.98% after hot water extraction, while the yield of gelatin dropped significantly with increasing duration of acid treatment. The results from differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FT-IR), and circular dichroism (CD) revealed that the triple-helical structure of collagen became progressively loose and valence bonds stabilizing the triple-helix were gradually destroyed during the process of acid treatment. The triple-helical structure was excessively destroyed after 8-h acid treatment, which could be the main reason for the decrease in gelatin yield. Moreover, the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) indicated that collagen peptide chains were degraded during acid treatment and the gelatin subunit content was directly influenced by the amount of subunits that remained after gelatinization. Therefore, during the gelatinization of collagen, a balance between subunit Degradation and the loosening of the triple-helical structure are required, in order to obtain high yield of high-quality gelatin.

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Original research was done by Wang Xuemeng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Porcine Skin Collagen Gelatinization Molecular Mechanismporcine Skin Collagen Gelatinization Molecular Mechanism

Abstract

This article uses pig skin collagen as raw material, and uses ultrahigh pressure and dilute hydrochloric acid to induce collagen gelatinization. The focus was on the effects of different induction times on the molecular mechanism of collagen gelatinization. Within the treatment time range of 5 min to 30 min, the thermal denaturation temperature Tm and heat enthalpy value rH of collagen first decreased and then increased, which was consistent with the change trend of gelatin yield. Infrared spectroscopy (FT-IR) results show that most of the triple helical structure is destroyed after ultrahigh pressure collaborative acid treatment, and the 15 min treatment causes the most severe damage to the secondary structure, which is consistent with the yield change trend, indicating that the degree of damage to the secondary structure of collagen is related to Gelatin yield related. SDS-PAGE analysis showed that the ultra-high pressure synergistic acid treatment process will degrade the subunit components of collagen, and there is no obvious difference in the degree of destruction of subunit components at different treatment times. Comparing the subunit component content of gelatinized collagen and corresponding gelatin, it was found that The degree of degradation of subunit components after late heat treatment was very low, indicating that ultrahigh pressure synergistic acid treatment inhibited excessive degradation of subunit components during heat treatment. Ultra-high pressure (UHP) with dilute hydrochloric acid (HCl) was used to induce the gelatinization of collagen derived from porcine skin. The effects of various processing times on the molecular mechanism of collagen gelatinization were investigated. The denaturation temperature (Tm) and enthalpy (rH) of gelatinized collagen initially decreased followed by an increase as the processing time increased from 5 to 30 min, which also corresponded with the increased gelatin yield. FT-IR showed that the triple helix in the gelatinized collagen was almost completely damaged. The secondary structures were partly retained and the highest amount of damage was observed after 15 min of treatment. This was also consistent with the gelatin yield, which possibly imply that the secondary structures of collagen are correlated with the gelatin yield. SDS-PAGE analysis indicated that the subunit compositions of collagen were degraded during the UHP in presence of dilute HCl, but the extent of damage was similar in treatments that were conducted for 5 to 30 min. Compared with the gelatinized collagen, less subunit compositions were damaged during the heating treatment , which indicated that the treatment via a combination of UHP and h HCl could inhibit the degradation of subunits during subsequent heating treatment.

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Original research was done by Yu Wei

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Tilapia Skin, Collagen Peptides, Intestinal Tumors, Reactive Oxygen Species, Mitochondrial Respiratory Chain Complex

Abstract

Objective: To study the effect of tilapia skin collagen peptide on the proliferation ability of human colon cancer cell line LoVo in vitro, and to preliminarily explore its mechanism of action. Methods: Human colon cancer LoVo cells were cultured in vitro, and the effect of tilapia skin collagen peptide on cell proliferation was detected by methylthiazolyldiphenyl-tertrazolium bromide (MTT) method; changes in cell membrane and nucleus were observed by fluorescent probe staining; laser confocal The expression of reactive oxygen species (ROS) in cells was observed under a microscope; the activities of mitochondrial respiratory chain enzyme complexes I and III were detected by colorimetry. Results: Tilapia skin collagen peptide had a certain inhibitory effect on the proliferation of LoVo cells. When the mass concentration was 100 mg/mL and the action was for 48 hours, the inhibition rate reached 55.03% (P<0.05); compared with the blank control group Ratio, after the action of collagen peptides, the nucleus of LoVo cells was concentrated, the cell membrane appeared incomplete, and the cells were apoptotic; the expression of intracellular reactive oxygen species was significantly increased (P<0.05), and the activity of intracellular mitochondrial respiratory chain complex III was significantly reduced (P <0.05), but has no effect on the activity of mitochondrial respiratory chain complex I. Conclusion: Tilapia skin collagen peptide has a certain inhibitory effect on the proliferation of human colon cancer cells, which may be related to inhibiting the activity of mitochondrial respiratory chain complex III, increasing intracellular ROS levels, and damaging the cell membrane morphology. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Xiaoling, Yang Li, Jiang Hongrui, Bai Yang, Mi Shunli, Shao Yong  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Type Ii Collagen, Immunoactive Peptide, Neutral Protease, Lymphocyte Proliferation Activity

Abstract

Using the hydrolysis degree as an indicator, the conditions for the enzymatic hydrolysis of chicken breast cartilage type II collagen by neutral protease were optimized. On this basis, type II collagen enzymatic hydrolysis complexes with different hydrolysis degrees were prepared, and their immune activity was evaluated using the lymphocyte proliferation rate as an indicator. Research. The results show that neutral protease has the strongest enzymatic hydrolysis ability, and its optimal enzymatic hydrolysis conditions are: enzymatic hydrolysis temperature 50 ¡æ, pH 7.5, substrate mass concentration 25 mg/mL, enzyme addition amount 40 U/mg, enzymatic hydrolysis time 150 minutes. When the hydrolysis degree of type II collagen is 18%, its proliferation activity on lymphocytes reaches 58.69%. The mass concentration of type II collagen enzymatic complex also has a significant impact on the proliferation rate of lymphocytes. When its mass concentration reaches 1 mg/mL, the lymphocyte proliferation ability reaches the maximum. The molecular weight of type II collagen enzymatic complex with high immune activity is mainly distributed in the range of 1 000 to 180 D, accounting for 75.21% of its total content.

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Original research was done by Yu Jinsong, Liu Lulu, Xu Fei, Cao Hui, Yuan Min

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.