Collagen series

Keywords

Matrix Metalloproteinase-2, Matrix Metalloproteinase-9, Breast Cancer, Type Iv Collagen, Immunohistochemistry

Abstract

Objective To study the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in breast ductal carcinoma and their relationship with type IV collagen (collagenIV). Methods Immunohistochemical double staining method was used to detect the expression of MMP-2, MMP-9 and type IV collagen in 60 cases of breast ductal carcinoma and analyze their correlation with tumor size, lymph node metastasis and human epidermal growth factor receptor-2 (C -erbB-2) relationship. Results The positive expression rates of MMP-2 and MMP-9 in breast ductal carcinoma were 75% and 80% respectively, which were significantly higher than 10% in normal breast tissue; MMP-2 and MMP-9 were close to the basement membrane or would break through the basement membrane. The expression of MMP-2 and MMP-9 was significantly enhanced in cancer cells at the membrane. %, 85.1%) expression rate was significantly higher than that of the corresponding control group (58.6%, 65.5%), (64.9%, 67.6%), (46.2%, 53.8%); the expression of type IV collagen was related to MMP-2, MMP- 9 expressions were all negatively correlated. Conclusion In the process of local invasion and distant metastasis of breast ductal carcinoma, MMP-2 and MMP-9 may play an important role in the degradation of type IV collagen. The joint detection of MMP-2, MMP-9 and type IV collagen may play an important role in the judgment of breast ductal carcinoma. It has reference value for the invasive and metastatic potential of breast cancer and for assessing prognosis.

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Original research was done by Su Shujuan, Xing Luqi, Chen Dengting, Deng Miao

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Abstract

Study the selective anti-tumor effect of lidamycin (ldm) and anti-type IV collagenase monoclonal antibody 3g11 immunoconjugates prepared by different coupling methods. Using spdp and smbs as coupling agents, the lysine at position 69 of the LDM prosthetic protein was connected to the 2-iminotetrahydrothiophene-modified monoclonal antibody 3g11 to prepare an immunoconjugate; the immune activity of the conjugate was measured by ELISA. The anti-tumor effects of the two conjugates were observed by cloning formation method and human ht-1080 cell immunodeficiency mouse transplantation model. The conjugate retained the immune activity of monoclonal antibody 3g11 against type IV collagenase, and the killing effect of 3g11-smbs-ldm on HT-1080 cells cultured in vitro was stronger than that of ldm and 3g11-spdp-ldm. Animal experiments show that the tumor inhibition rate of ldm under equimolar dosage conditions is 86.1%, the free ldm group is 71.2%, the 3g11-spdp-ldm group is 77.1%, the inhibitory effect of 3g11-smbs-ldm Significantly enhanced. The average survival time of animals in the 3g11-smbs-ldm group was extended to 163.7%, that of the 3g11-spdp-ldm group was 125.3%, and that of the ldm group was 71.9%. 3g11-smbs-ldm can significantly extend the survival time of tumor-bearing mice. There are significant differences between the two species. The 3g11-smbs-ldm conjugate has more selective anti-tumor effects, significantly improved efficacy, prolonged survival of tumor-bearing animals, and significantly reduced toxicity, and may become a new tumor treatment drug.

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Original research was done by Feng Yun, Zhen Yongsu, Dai Yao, Shang Boyang, Zhang Min, He Hongwei, Li Baobo, Shao Rongguang

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Lignin, Collagen, Green, Composite, Puffing, Inhibition

Abstract

Biomass solid waste such as lignin and collagen have the potential to replace coal pitch binders in the preparation of carbon products. The control of their puffing behavior during high-temperature pyrolysis is a key factor affecting the physical and chemical properties of carbon products. For this reason, pine wood was used The lignin/collagen blend system was used as the binder, and the graphite electrode green body was prepared using hot pressing technology. The impact of the roasting process and puffing inhibitors on the puffing behavior during the roasting process was investigated, and through elemental analysis and scanning electron microscopy (SEM) ) for analysis. The results show that reducing the heating rate (0.04 ¡æ/min in the range below 500 ¡æ, 0.07 ¡æ/min in the range of 500~800 ¡æ), and appropriately increasing the cooling rate (1.08 ¡æ/min) is beneficial to inhibiting the roasting process. The generation of medium swelling behavior; Swelling inhibitors such as coconut shell charcoal, lignite, boric acid, ferric oxide and ferric chloride are difficult to apply to the swelling inhibition of biomass bonding systems. As the swelling inhibition effect increases, the appearance of carbonized electrodes The density shows a gradual attenuation trend; in the graphite electrode green system containing iron oxide, as the pyrolysis temperature increases, the C element continues to enrich, the mass fraction of N, O, and S elements continues to decrease, and the mass fraction of Fe element continues to decrease. It shows a fluctuating change. The pine wood lignin/collagen bonded coke gradually consolidates in the form of surface adhesion, internal filling or bridging, and finally forms a “graphite-like structure” coke. In the graphitization stage, the Fe element is deposited in the form of granular elemental iron. on the coke surface. Biomass solid wastes, such as lignin and collagen, are potentialized to be developed as coal tar pitch alternatives to prepare the carbon products. However, controlling the puffing behavior during the carbonization is a key factor to obtain the excellent carbon materials. In the present study, green composites using the pine softwood lignin and collagen as hybrid binder were prepared by the hot-pressing process. The effect of baking program and puffing inhibitors on the puffing behavior was evaluated. Elemental analysis and Scanning Electronic Microscopy (SEM) were also employed for further study. Results showed that a lower heating rate (baking rate of 0.04 ¡æ/min to 500 ¡æ, then 0.07 ¡æ/min to 500-800 ¡æ) and a higher cooling rate (1.08 ¡æ/min) were preferred for puffing inhibition. Some traditional puffing inhibitors, such as coconut shell, lignite coal feed, boric acid, ferric oxide, and iron (III) chloride, were not available to the bio-binder system. Along with the improvement of puffing inhibition, the apparent density of the baked product gradually decreased. In the case of a mixture system containing ferric oxide, the rising temperature promoted the enrichment of carbon element and the decrease of other elements such as N, S, and O, resulting in fluctuating change in Fe content. The lignin/collagen hybrid system served as thermally-fused binder to form a new integrated char with turbostratic structure by means of covering, filling and bridging, and iron precipitates derived from ferric oxide was observed on the surface of char during the graphitization.

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Original research was done by Zhao Zilong, Wang Hongjie, Dong Wenyi

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Phenytoin, Wound Healing Rate, Type I Collagen, Vascular Endothelial Growth Factor, Bcl-2, Bax

Abstract

Objective To observe the effect of phenytoin on the wound healing process in experimental rats and explore its mechanism of action. Methods 100 SD rats were randomly divided into 5 groups, namely blank control group, lithospermum oil positive control group, phenytoin sodium low-dose group (5 mg/cm~2), and medium-dose group (10 mg/cm~2). , high dose group (20 mg/cm~2), 20 animals in each group. A 2 cm ¡Á 3 cm rectangular wound model was designed on the dorsal side of the rat spine. The wound healing rate was observed on days 4, 8, 12, and 16 after trauma, and immunohistochemistry was used to detect the expression of type I collagen, vascular endothelial growth factor (VEGF), Bcl-2, and Bax in the wound. Results The wound healing rate of the low-dose phenytoin group was significantly better than that of the blank control group and the medium- and high-dose phenytoin groups at 8, 12, and 16 days after trauma (P<0.05). The positive expression of type ¢ñ collagen, VEGF, Bcl-2, and Bax showed a gradually increasing trend. The expression of Bax increased most obviously in the phenytoin sodium medium and high-dose groups, and the difference between the high-dose group and the low-dose group, blank control group, and lithospermum oil group was statistically significant at 16 days after trauma (P<0.05). The expression of type ¢ñ collagen increased the least in the high-dose phenytoin sodium group, and there was a statistically significant difference between the high-dose group, the low-dose group, the blank control group, and the lithospermum oil group 16 days after trauma (P<0.05). On the 4th day after trauma, the expression of VEGF in the phenytoin sodium low-dose group increased significantly. On the 16th day, the expression of VEGF in the lithospermum oil positive control group and the phenytoin sodium low-dose group had no statistical significance, but there were differences compared with other groups. Statistical significance (P<0.05). On the 4th day after trauma, the expression of Bcl-2 in the phenytoin sodium low-dose group and the lithospermum oil group increased simultaneously, and was still higher than that in the other groups on the 16th day (P<0.05). The expression of the phenytoin sodium high- and medium-dose groups was lower than that in the blank control group. (P<0.05). Conclusion The low-dose phenytoin sodium group can promote wound healing by promoting fibroblast proliferation, vascular regeneration, and extracellular matrix deposition. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Jun, Zhu Lijuan, Liu Jianyun, Deng Jinju, Zhou Junli  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Guizhi Warms And Regulates Meridians, Inflammatory Cytokines, Rheumatoid Arthritis

Abstract

Objective To study the therapeutic effect of Guizhi warming and dredging meridians on collagen-induced arthritis (CIA) model rats, and to explore its mechanism of action. Methods 110 male Wistar rats were randomly divided into 10 normal groups. The remaining rats were injected intradermally with 0.5 mg of collagen emulsion into the tail root and back of each rat to prepare an arthritis rat model. 21 days after the inflammation was induced, the model was successfully modeled. Rats (90 rats) were randomly divided into model group, methotrexate group, Guizhi group, Guizhi Achyranthes group, Guizhi Chuanwu group, Guizhi Duhuo group, Guizhi Aconite low, medium and high-dose groups. Group of 10 animals; administered intragastrically once a day for 21 consecutive days. The arthritis index (AI), hind paw volume and ankle joint pathological changes of rats were observed, and the serum levels of tumor necrosis factor-¦Á (TNF-¦Á), interleukin-1¦Â (IL-1¦Â), and interleukin-¤B6 (IL) were detected. -6) content. Results Compared with the model group, the AI and hindpaw swelling of arthritic rats in the Guizhi Achyranthes group, Guizhi Aconite group, Guizhi Duhuo group, Guizhi Aconite high and medium dose groups and methotrexate group were significantly reduced (P< 0.05); the serum TNF-¦Á content of arthritic rats in the Guizhi Achyranthes group, Guizhi Chuanwu group, Guizhi Duhuo group, Guizhi Aconite medium and high-dose groups and methotrexate group was significantly reduced (P<0.01) and improved the pathological changes of the ankle joint; the serum IL-¤B6 content of rats in each administration group was significantly reduced (P<0.01). Conclusion The combination of Guizhi warming and dredging meridians has a certain inhibitory effect on joint inflammation in CIA rats, and may be an effective combination in the treatment of rheumatoid arthritis. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Zhou Ruibin, Peng Daiping, Tang Xiaohu  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Mycobacterium Tuberculosis Rv3194C Protein Hyaluronic Acid Chondroitin Sulfate Type I Collagen

Abstract

[Purpose] The Rv3194c gene encodes the PDZ signaling protein of Mycobacterium tuberculosis. This study explored whether it has adhesin properties. [Method] Rv3194c was expressed prokaryotically, and then the Rv3194c protein was incubated with hyaluronic acid, chondroitin sulfate, and type I collagen in buffers at different temperatures (37, 38, 39, 40¡ãC) overnight, and then used Western blot and ELISA to analyze the changes in component content in the supernatant. [Results] Western blot identification results showed that His-Rv3194c protein was expressed in a soluble form, and the molecular mass of the protein was 35 kDa. Western blot showed that the His-Rv3194c protein content in the supernatant of the 39¡ãC experimental group (***P<0.001) was significantly smaller than that of other experimental groups (37, 38, 40¡ãC); ELISA showed that the content of His-Rv3194c protein in the supernatant of the 39¡ãC experimental group The contents of hyaluronic acid (HA), chondroitin sulfate (CS), and type I collagen (Collagen I) (***P<0.001) were extremely significantly lower than those of other experimental groups (37, 38, and 40¡ãC). [Conclusion] It was confirmed for the first time that Rv3194c protein has adhesin properties and can become a target protein for the development of new anti-tuberculosis drugs. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Lin Lili, Wen Fuli, Zhao Dongyue  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Silk Fibroin, Collagen, Hydroxyapatite, Mc3T3-E1 Cells, Mechanical Loading

Abstract

Objective To observe the effect of dynamic mechanical loading on the proliferation, differentiation and osteogenesis-specific gene expression of MC3T3-E1 cells in the three-dimensional biomimetic composite scaffold material prepared by low-temperature 3D printing combined with freeze-drying method. Methods Silk fibroin, type I collagen, and hydroxyapatite were mixed at a mass ratio of 3:9:2, and a three-dimensional biomimetic composite scaffold was prepared using low-temperature 3D printing combined with freeze-drying technology. The appearance of the scaffold was generally observed, and the pore size of the scaffold was observed with Micro-CT. and porosity, and determine the water expansion rate, stress, strain, and elastic modulus. MC3T3-E1 cells were seeded into a three-dimensional biomimetic composite scaffold and randomly divided into two groups: the experimental group underwent dynamic mechanical loading once a day, 15 minutes each time, frequency 1 Hz, and strain 3 500 ¦Ì¦Å during culture; the control group received no mechanical loading. After 7 and 14 days of culture, HE staining and scanning electron microscopy were performed on the cell-scaffold complex to observe the cell growth on the scaffold. Western blot and real-time fluorescence quantitative PCR were used to detect type I collagen, BMP-2, and osteocalcin (OCN). ) protein and mRNA expression. As a result, the three-dimensional bionic composite scaffold prepared was a white cubic grid. Micro-CT examination showed that the interior of the scaffold material had a pore network structure with good pore connectivity; the macropore diameter was (506.37¡À18.63) ¦Ìm and the micropore diameter was (62.14¡À 17.35) ¦Ìm, the porosity is 97.70%¡À1.37%, and the water expansion rate is 1341.97%¡À64.41%. Mechanical testing shows that when the stent is compressed to 10%, the compression displacement of the stent is (0.376¡À0.004) mm, the compressive stress is (0.016¡À0.002) MPa, and the elastic modulus is (162.418¡À18.754) kPa. After 7 and 14 days of composite culture, HE staining and scanning electron microscopy of both groups showed that cells grew inside the scaffold, mainly distributed around the pore walls of the scaffold. The cells in the experimental group increased compared with the control group, and the cells changed from spindle-shaped to flat. Except for the difference in cell count between the two groups at 14 days under 200x microscope (t=¨C2.024, P=0.080), the cell numbers of the experimental groups at each time point under different magnifications (40, 100, and 400x) were all the same. Significantly higher than the control group (P<0.05). Real-time fluorescence quantitative PCR detection showed that the relative expression of type I collagen and OCN mRNA in the experimental group was significantly higher than that in the control group at 7 and 14 days of culture (P<0.05), but there was no statistical difference in the relative expression of BMP-2 mRNA between the experimental group and the control group. scientific significance (P>0.05). Western blot analysis showed that the relative expression levels of type I collagen, BMP-2, and OCN proteins in the experimental group were significantly higher than those in the control group (P<0.05) at 7 and 14 days of culture. Conclusion After dynamic mechanical loading, the expression of BMP-2, type I collagen, and OCN in MC3T3-E1 cells seeded on the three-dimensional biomimetic composite scaffold was significantly increased, indicating that appropriate mechanical loading is conducive to the differentiation of MC3T3-E1 cells into osteoblasts. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Liu Yingjie, Zhang Xizheng, Li Hui, Li Ruixin, Cheng Wei, Yuan Qingxian  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Ordered Materials, Spinal Cord Injury, Collagen Binding Domain-Brain Derived Neurotrophic Factor, Bbb Score, Inclined Plane Score, Uniaxial Guidance Collagen Scaffolds

Abstract

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Original research was done by CHEN Lei, LIU Dongxiao, LI Kaiming, SONG Xinqiang, ZENG Xiansi, JIANG Lijie

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Sclera,Collagen,Fibrils,Fibroblasts,Elastic,Modulus

Abstract

Objective To explore the changes in morphological structure and biomechanical properties of rabbit scleral tissue at different ages. Methods: New Zealand white rabbits aged 1, 2, and 3 months were selected, the eyeballs were removed, and the diameter and axial length of the eyeballs were measured. The scleral tissue was obtained, and the thickness of the scleral tissue was measured. After a part of the scleral tissue was fixed, HE staining was performed to observe the scleral structure. After a part of the scleral tissue was fixed, the scleral structure was observed. The collagen fibrils were observed through an electron microscope, and another part of the scleral tissue was made into a scleral strip, and the elastic modulus of the sclera was measured using an Instron 5544 testing machine. Results: Eyeball diameter, axial length and scleral thickness increased with age. The elastic modulus of scleral tissue increases with age. The number of scleral fibroblasts in the scleral tissue decreases with age, and the fiber bundles increase and become thicker. The diameter of collagen fibrils increases with age. Conclusion During the postembryonic growth of sclera, the structures of the eyeball and sclera continue to change, collagen fibers continue to increase and thicken, and the biomechanical properties of scleral tissue also improve. Understanding the mechanism of postembryonic growth and development of sclera can provide guidance for the treatment of scleral-related diseases. Provide theoretical guidance for prevention and treatment. Objective To investigate the changes of morphological structures and biomechanical properties of scleral tissues in rabbits at different month ages. Methods The eyeballs of 1, 2 and 3 month-old New Zealand white rabbits were obtained for measuring the diameter and axial length, and the thickness of scleral tissues. Part of the scleral tissues was used to observe scleral structures with HE staining, some other part was used to observe collagen fibrils by electron microscope, and the left part were cut into strips and used to test the elastic modulus of the sclera on Instron 5544 system. Results The diameter, axial length of eyeballs and the thickness of the sclera were increased with month age. The elastic modulus of scleral tissues was also increased with month age. The numbers of scleral fibroblasts were decreased and the numbers of fiber bundles were increased with month age. The diameters of collagen fibrils were increased with month age. Conclusions In the post-embryonic stages, the structures of eyeball and sclera are changed continually, with growing numbers of thicker collagen fibers, and the biomechanical properties of scleral tissues are improved correspondingly. The mechanism of post-embryonic development in sclera is further explained in the study, which can provide theoretical guidance for prevention of sclera-related diseases.

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Original research was done by Hao Shuang, Deng Aijun, Wang Guohui

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.

Keywords

Amniotic Membrane, Bone And Bone Tissue, Biomedical Engineering, Collagen Type I, Alkaline Phosphatase

Abstract

? Purpose: To initially explore the potential of human amniotic membrane stromal cells (hamc) as seed cells for bone tissue engineering. Methods: hamc was isolated and cultured, and the third generation hamc was induced into osteogenesis in DMEM culture medium containing dexamethasone (0.1 ¦Ìmol/l), vitamin C (50 mg/l) and ¦Â-glycerol sodium phosphate (10 mmol/l). After culturing for 1 week, alizarin red staining was performed to count the number of calcified nodules, and immunofluorescence cytochemical staining was performed to detect the expression of type I collagen and alkaline phosphatase. Immunohistochemical staining was used to detect the expression of fasl in amniotic membrane and hamc. Results: After osteogenic induction of hamc, obvious calcified nodules were formed, with an average of 18 per well. The cells in the calcified nodules expressed positive expression of type I collagen and alkaline phosphatase. The positive expression of fasl can be detected in cells in human amniotic membrane tissue and in hamc cultured in vitro. Conclusion: hamc has the potential to become an ideal seed cell for bone tissue engineering.

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Original research was done by Mei Fang, Tang Junmin, Zhong Jinyan, Qi Weihong, Tang Yan, Li Feng

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.