Screening And Phylogenetic Analysis Of A Collagenase-Producing Bacterium
Keywords
Bacillus Megaterium, Collagenase, 16Srdna Sequence, Phylogeny
Abstract
From the collected soil and water samples, a Gram-positive strain with a columnar spore-producing number numbered LY-t1 was isolated and screened. By conducting a cowhide digestion experiment on this bacterium, and using type I collagen as a substrate, the strain was determined. The collagenase activity in the bacterial fermentation culture medium was confirmed to have collagenase activity. 16S rDNA sequence analysis showed that the isolated strain was 98% similar to Bacillus megaterium. Finally, the PHYLIP program was used to compare this strain with the reported related collagenase-producing bacteria. Perform phylogenetic evolutionary analysis.
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Original research was done by Liu Guilan, Fang Baishan, Wang Geng, Jin Hongwei
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