Combined Effects Of Methionine Restriction And Collagen Peptides On Lipid Metabolism And Oxidative Stress In Mice Fed A High-Fat Diet
Keywords
Dietary Methionine Restriction, Collagen Peptides, Joint Effects, Lipid Metabolism, Oxidative Stress
Abstract
Objective: To study the combined effects of dietary methionine restriction (MR) and collagen peptides on hepatic lipid metabolism and oxidative stress in mice fed a high-fat diet. Methods: 36 C57BL/6 male mice were randomly divided into 4 groups: low-fat normal diet group (ND group), high-fat diet group (HF group), high-fat methionine restriction group (MR group) and high-fat methionine restriction group and collagen peptide combined effect group (PMR group). Body weight was monitored weekly. At the end of the 22-week experiment, plasma triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and free fatty acids (free fatty acids) were measured. acid, FFA) content; determine the TG and TC content in the liver; determine the total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px) in the liver and plasma ) activity and malondialdehyde (MDA) content; real-time fluorescence quantitative polymerase chain reaction (quantitative real time polymerase chain reaction, qPCR) determination of lipid metabolism-related genes sterol regulatory element-binding protein 1c (sterol regulatory element-binding protein) 1c, SREBP1c), fatty acid synthase (FAS), acetyl CoA-carboxylase 1 (ACC-1), cholesterol 7¦Á-hydroxylase (CYP7A1), right Expression of carnitine palmityl transferase 1 (CPT1) and peroxisome proliferator activated receptor ¦Á (PPAR¦Á). Results: Compared with the HF group, the body weight of mice in the MR group and the TG and TC contents in the liver were significantly reduced (P<0.01); the liver fat synthesis-related genes FAS and SREBP1c were extremely significantly down-regulated (P<0.01), and ACC- 1 was significantly down-regulated (P<0.05); the lipolysis-related genes CYP7A1 and PPAR¦Á were significantly up-regulated (P<0.01), and CPT1 was significantly up-regulated (P<0.05); the activities of T-AOC and GSH-Px in the liver were significantly increased (P<0.05 ), the contents of MDA and reactive oxygen species were significantly reduced (P£¼0.01); the activities of T-AOC and GSH-Px in plasma were extremely significantly increased (P£¼0.01), and the contents of plasma MDA and whole blood reactive oxygen species were extremely significantly reduced (P£¼0.01) ). Compared with the MR group, the body weight and TG content in the liver of the PMR group were extremely significantly reduced (P<0.01); the TG and TC content in plasma were significantly reduced (P<0.05); the fat synthesis-related genes SREBP1c and ACC-1 were significantly reduced. Down-regulation (P<0.05); lipolysis-related genes CPT1 and PPAR¦Á were significantly up-regulated (P<0.05); liver GSH-Px activity was significantly increased (P<0.05). Conclusion: Methionine restriction diet can significantly reduce the accumulation of blood lipids and liver fat in mice on a high-fat diet, and enhance the antioxidant capacity of the liver. The combined effect of methionine restriction and collagen peptides can significantly reduce the accumulation of blood lipids and liver fat in mice on a high-fat diet. Enhance liver antioxidant capacity For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Le Guowei, Zhang Jiahong, Shi Yonghui, Wang Yanan, Guo Haitao
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