Comparative Study On Enzyme-Soluble Collagen From Several Freshwater Fish Scales
Keywords
Freshwater Fish Scales, Enzyme-Soluble Collagen, Sds-Page Electrophoresis, Fourier Transform Infrared Spectroscopy
Abstract
Infrared spectroscopy (FT-IR) was used to analyze and compare the effects of three different pretreatment reagents on the structure of enzyme-soluble collagen (PSC) from the scales of several freshwater fish. The results show that hydrochloric acid and sodium carbonate have a strong destructive effect on the structure of fish scale PSC; the denaturation temperatures of four freshwater fish scale PSCs, grass carp, bighead carp, silver carp and crucian carp, measured using an Ubbelohde viscometer, are 33.0, 31.9, 31.5, respectively. 30.8¡æ; the hydroxyproline content in 4 kinds of fish scale PSC was measured to be 47.85, 44.91, 40.36, 39.59 mg/g respectively; the molecular weight distribution of 4 kinds of fish scale PSC was examined by electrophoresis, among which grass carp scale PSC consists of 3 different peptide chains Composed of ¦Á1, ¦Á2, and ¦Á3; bighead carp, silver carp, and crucian carp are composed of two ¦Á chains (¦Á1 and ¦Á2); the electrophoresis band has no miscellaneous bands, indicating that the purity of the extracted collagen is high and electrophoretically pure; comparative analysis by infrared spectrum Four kinds of fish scale PSC structures were identified, and the results showed that the infrared structures of the four kinds of fish scale PSCs were basically the same. Combined infrared spectroscopy and electrophoresis analysis showed that the four types of fish scale PSCs were type I collagen.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Wan Qionghong, Dang Meizhu, Zeng Jiancheng, Li Chunmei, Zhao Honglei
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
