Comparative Study On The Extraction Of Collagen From Pig Skin By Several Enzymatic Methods
Keywords
Collagen, Enzymatic Hydrolysis, Hydroxyproline, Extraction
Abstract
The extraction of collagen from pig skin using trypsin, papain and pepsin was studied. Research has found that the protein content of pig skin is approximately 24.59% and the hydroxyproline content is 2.3%. After the pig skin is enzymatically hydrolyzed, a crude collagen extract is obtained. The crude extract is then dialyzed, concentrated, and finally freeze-dried to obtain collagen products with higher purity. The results of the study show that three types of enzymes were used to treat pig skin. Among them, the trypsin extract had the highest yield, but the structure was seriously damaged. The collagen product extracted with pepsin had the most complete structure and the whitest color, but the lowest yield.
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Original research was done by Li Yan, Wang Chuan, Lan Weiqing, Ma Zhiying
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