Comparison Of Three Human-Like Collagen Determination Methods
Keywords
Coomassie Brilliant Blue Method, Folin, Phenol Method, Biuret Method, Human-Like Collagen, Protein Content Determination
Abstract
Using the Kjeldahl method as the benchmark method, the content of human-like collagen extracted from the recombinant Escherichia coli fermentation product was determined using the Coomassie brilliant blue method, the folin-phenol method, and the biuret method. The results show that there are differences in the characteristics of human-like collagen, bovine serum albumin, and gelatin. Among the three colorimetric analysis methods, Coomassie brilliant blue method, folin-phenol method, and biuret method, the biuret method was used. The regression equation coefficients of human-like collagen and bovine serum albumin have the smallest difference, and the correction coefficients ¦Á and ¦Â of the regression equation are obtained, where ¦Á represents the difference in characteristics between human-like collagen and bovine serum albumin (¦Á=0.6761), ¦Â It is the percentage of protein in the human collagen sample (g/g).
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Original research was done by Luo Yan’e, Fan Daidi, Liu Shuwen
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