Determination And Recovery Of Squid Skin Collagen
Keywords
Hydroxyproline, Collagen, Squid Skin, Determination, Recovery
Abstract
In order to recover beneficial components such as collagen in squid skin, the quantitative method of collagen was first investigated, and a method suitable for the determination of collagen content in squid skin (using hydroxyproline juice) was established. The results show that when measuring the collagen content in squid skin, it is advisable to degrease the squid skin first and then directly hydrolyze it to determine hydroxyproline. In terms of hydrolysis conditions, 120¡ãC hydrolysis for 12 hours or 130¡ãC hydrolysis for 6 hours can be used instead of 105¡ãC for 16 hours in the iso-3496e method to shorten the hydrolysis time. Secondly, enzymatic solubilization and hot water extraction methods are used to treat squid. Collagen in the skin is recycled. Based on the collagen recovery rate, it is proposed that pepsin enzymatic hydrolysis at 5¡ãC for 72h and water extraction at 100¡ãC for 6h are enzymatic extraction and hot water extraction methods to recycle the collagen in squid skin. , based on the collagen recovery rate, it was concluded that the optimal conditions for enzymatic extraction with pepsin at 5¡ãC for 72 hours and water extraction at 100¡ãC for 6 hours were respectively the optimal conditions for enzymatic extraction and hot water extraction. The extract was prepared and then subjected to sensory identification. The statistical results showed that , the pepsin extract was significantly better than the 100¡ãC water extract. The selection of pepsin enzyme extraction temperature shows that the pepsin dissolving temperature should be controlled below 15¡ãC to obtain higher collagen recovery rate and better color of the enzyme extraction liquid.
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Original research was done by Qin Yuqing Liu Chengchu Wang Zhen
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