Determination Of Chloramphenicol Residues In Propolis Collagen By High Performance Liquid Chromatography-Tandem Mass Spectrometry
Keywords
Propolis Collagen Chloramphenicol High Performance Liquid Chromatography-Tandem Mass Spectrometry (Hplc-Ms/Ms)
Abstract
An analytical method for the determination of chloramphenicol residues in propolis collagen by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. Dissolve the sample with tert-butyl methyl ether, remove flavonoids and other impurities with sodium hydroxide solution, add n-hexane to the tert-butyl methyl ether layer to reduce the solubility of chloramphenicol, and then back-extract chloramphenicol with sodium acetate buffer, and adjust the back-extraction solution to alkaline It was then extracted with ethyl acetate, concentrated, reconstituted and filtered before measurement. Use methanol-water (65:35, volume ratio) as the mobile phase, reverse-phase Atlantis T3 chromatographic column for liquid chromatography separation, electrospray negative ion ionization (ESI-), multiple reaction monitoring mode (MRM) for detection, and internal standard method. Quantitative. The results show that chloramphenicol has a good linear relationship in the range of 0.1~5.0 ¦Ìg/L; the lower limit of quantification (S/N¡Ý10) of the method is 0.3 ¦Ìg/kg; at the spike levels of 0.3, 0.6, and 3.0 ¦Ìg/kg, The average recovery rate of chloramphenicol was 97.3%~103%, and the relative standard deviation was 4.8%~6.4%. The sensitivity, accuracy and precision of this method all meet the requirements for veterinary drug residue detection
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Original research was done by Lin Feng, Xie Minling, Shao Linzhi
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