Dual Effect Of Mechanical Strain On The Pelvic Fibroblast Cells
Keywords
Mechanical Force, Fibroblasts, Pelvic Organ Prolapse, Type I Collagen, Type Iii Collagen
Abstract
Objective: To explore the bidirectional effects of mechanical force on pelvic floor fibroblasts. Methods: The sacral cardinal ligament tissues from patients who underwent total vaginal hysterectomy for non-pelvic organ prolapse in our hospital from January 2013 to January 2015 were collected, primary cultured fibroblasts were grouped as follows according to different mechanical force loading parameters : (1) No force control group; (2) 1mm 4h0.5Hz group; (3) 4mm 4h0.5Hz group. CCK-8 method was used to detect the cell proliferation activity of each group after intensive intervention, and Western Blot was used to detect the expression of type ¢ñ and ¢ó collagen in the cells of each group. Results: After loading human pelvic floor fibroblasts with different mechanical parameters, compared with the control group, the cell proliferation activity of the 1 mm 4h0.5 Hz group was enhanced (188.22% ¡À 10.53%), and the cell proliferation activity of the 4 mm 4h0.5 Hz group was weakened. (66.09%¡À4.06%), the differences were statistically significant (P<0.05). Western Blot detected changes in the expression levels of collagen types I and III in each group. Compared with the control group, the expression of collagen types I and III in human pelvic floor fibroblasts in the 1mm 4h0.5 Hz group was significantly increased (P<0.05 , P<0.001), while the expression of type I collagen in human pelvic floor fibroblasts in the 4mm 4h0.5Hz group was significantly down-regulated (P<0.001, P<0.05). Conclusion: Mechanical force has a bidirectional regulatory effect on pelvic floor tissue damage or repair. The mechanism may be that mechanical force has a bidirectional regulatory effect on pelvic floor fibroblasts to promote proliferation or damage, and bidirectionally regulates the metabolism of type ¢ñ and ¢ó collagen. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Li Bingshu, Hong Li, Cheng Liwei, Min Jie, Liu Yaodan, Tang Jianming
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