Effect Of Erythropoietin On Cardiac Fibroblasts Cultured In Vitro Induced By High Glucose Environment
Keywords
Erythropoietin Cardiac Fibroblasts High Glucose Type I Collagen Type Iii Collagen Alpha-Smooth Muscle Actin
Abstract
Objective To explore the effect of erythropoietin (EPO) on the proliferation and phenotypic transformation of neonatal rat cardiac fibroblasts (CF) cultured in vitro during high-glucose environment induction. Methods Primary cells of suckling mice were used to culture CF. In the process of promoting the phenotypic transformation of CF cells, high-glucose medium was used for induction and EPO was added for intervention. The same batch of CF cells were randomly divided into 3 groups: control group (5.5 mmol/L glucose), high glucose group (25 mmol/L glucose), and high glucose + EPO group (25 mmol/L glucose, 20 kU/L EPO). Immunohistochemistry was used to identify CF, enzyme-linked immunosorbent assay was used to detect the content of type I and type III collagen, and Western blot was used to detect the expression of fibrosis indicator ¦Á-smooth muscle actin (¦Á-SMA). Results Immunohistochemical staining results were positive for fibronectin and vimentin and negative for ¦Á-actin, confirming that the cultured cells were cardiac fibroblasts. Compared with the control group, the expression levels of type ¢ñ and type ¢ó collagen in the high-glucose group were significantly increased (P<0.01), while the expression levels of type ¢ñ and type ¢ó collagen in the high-glucose + EPO group were significantly lower than those in the high-glucose group (P<0.01). . The expression of ¦Á-SMA in the high-glucose group was significantly higher than that in the control group (P<0.05), while the expression of ¦Á-SMA in the high-glucose + EPO group was significantly lower than that in the high-glucose group (P<0.05). Conclusion High-glucose environment can effectively induce phenotypic transformation of CF, and EPO can reverse myocardial fibrosis induced by high-glucose environment. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Jiang Zhihua, Zhang Xinjin, Li Jianmei, Luo Nanfang
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