Effect Of Fish Skin Collagen Peptide On The Growth Of Human Skin Keratinocytes
Keywords
Collagen Hydrolysates Tilapia Skin Shark Skin Antioxidant Activities Cell Viability Keratinocytes Collagen Hydrolysates Tilapia Skin Shark Skin Antioxidant Activities Cell Growth Keratinocytes
Abstract
This article examined the effects of shark skin collagen enzymatic hydrolyzate (SH), tilapia skin collagen enzymatic hydrolyzate (TH) and their isolated components on the growth of human skin keratinocytes (HaCat cells). The results showed that in the logarithmic phase of cell growth, adding 0.05 mg/mL or 0.20 mg/mL SH could significantly promote the proliferation of HaCat cells. However, when the concentration of SH was increased to 1.00 mg/mL, HaCat cells did not show significant proliferation. . Although TH can also promote HaCat cell proliferation, TH concentration has no significant effect on cell proliferation. On the other hand, whether it is shark skin collagen enzymatic hydrolyzate or tilapia skin collagen enzymatic hydrolyzate, the components separated by Sephadex G-15 gel chromatography have higher properties than the single-component Gly-Tyr dipeptide and tyrosine. It has better ability to promote cell proliferation and is even better than glutathione in inhibiting the production of MDA and increasing the SOD activity of cells. These results show that the components separated by gel chromatography from the enzymatic hydrolyzate prepared from fish skin collagen have superior ability to promote the growth of human skin keratinocytes and have the potential to replace glutathione in cosmetic applications as an antioxidant peptide. This study investigated the effects of shark skin collagen hydrolysates (SH), tilapia skin collagen hydrolysates (TH), and their isolated fractions on the viability of human skin keratinocyte (HaCat). The HaCat cell viability, determined with the MTT method, was increased notably with the addition of 0.05 mg/mL or 0.20 mg/mL SH during the exponential growth phase. However, when the concentration of the added SH was increased to 1.00 mg/mL, no significant proliferation of HaCat was observed. The HaCat cell viability with the addition of TH was higher than that of the control (without the addition of TH), but TH concentrations did not significantly influence the viability. On the other hand, the HaCat cell viability with addition of the fractions, which were isolated from SH or TH by Sephadex G-15, was much higher than that with the addition of dipeptide (Gly-Tyr) or Tyr. Compared to the sample with added GSH, the MDA content was lower and the SOD activity was higher with the added isolated fractions irrespective of fish skin collagen hydrolysates. Based on these results, it was concluded that the fractions isolated from fish skin collagen hydrolysates by Sephadex G-15, can effectively promote the proliferation of HaCat and have great potential to replace GSH as antioxidant peptides in the cosmetics industry.
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Original research was done by Chen Jun
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