Effect Of Tengberry Decoction On Traf3/Nf-¦ªb Signaling Pathway In Synovium Of Rats With Collagen-Induced Arthritis
Keywords
Traf3/Nf-¦ªb Signaling Pathway In Collagen-Induced Arthritis Caused By Tengberry Decoction
Abstract
Objective To observe the effects of Tengberry Decoction on joint synovium and serum tumor necrosis factor receptor-associated factor 3 (TRAF3) and interleukin in rats with collagen-induced arthritis (CIA). -23 (interleukin-23, IL-23), interleukin-17 (interleukin-17, IL-17), nuclear factor-kappa B (NF-¦ÊB), tumor necrosis factor-¦Á (tumor necrosis factor-¦Á (TNF-¦Á) expression and ankle joint pathology, and explore the molecular mechanism of this prescription in inhibiting synovial immune inflammatory damage in rheumatoid arthritis (RA). Methods A CIA rat model was established. The model rats were randomly divided into model group, positive control group, Tengberry Decoction large, medium and small dose groups, with 6 rats in each group, and a normal group of 6 rats. The normal group and the model group were given deionized water 10 mL/(kg¡¤d) by gavage. The positive control group was given leflunomide 1.87 mg/(kg¡¤d) by gavage. Tengberry Decoction was given in large, medium and small doses. The groups were orally administered Tengberry Decoction containing 28, 14, and 7 g/(kg¡¤d) of crude drug, and the intervention was continued for 12 weeks. Real-time PCR, Western blot, and ELISA techniques were used to detect the mRNA and protein expression levels of TRAF3, IL-23, IL-17, NF-¦ÊB, and TNF-¦Á, and light microscopy was used to observe pathological changes in the ankle joint. Results Compared with the normal group, the TRAF3 mRNA transcription and protein expression levels in the model group were down-regulated (P<0.01), and the IL-23, IL-17, NF-¦ÊB and TNF-¦Á mRNA transcription and protein expression levels were up-regulated (P<0.01). Compared with the model group, TRAF3 mRNA transcription and protein expression levels were increased in the middle-dose Tengberry Decoction group (P<0.01, P<0.05), and IL-23, NF-¦ÊB, IL-17, and TNF were found in the large and medium-dose Tengberry Decoction groups. -¦Á mRNA transcription and protein expression levels were down-regulated (P<0.01), and the protein expression levels of NF-¦ÊB, IL-17, and TNF-¦Á in the Tengberry Decoction low-dose group were down-regulated (P<0.01). The pathological changes of the ankle joints of CIA rats in each dose group of Tengberry Decoction were improved to varying degrees. Conclusion The mechanism by which Tengberry Decoction improves synovial immune inflammatory damage in CIA model rats may be related to the regulation of TRAF3/NF-¦ÊB signaling pathway For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Bu Xiangwei, Meng Fengxian, Zhang Jianping, Zhang Honghong
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