Effect Of Ultrasonic Waves On The Removal Of Impurity Proteins In Fish Skin And Its Effect On The Fiber Structure Of Fish Skin
Keywords
Ultrasonic Collagen Crude Protein Pretreatment Structure Hiu Collagen Crude Protein Pretreatment Structure
Abstract
In order to avoid the shortcomings of traditional stirring methods for removing impurity proteins, such as high energy consumption, high solvent consumption, and high time consumption, this study explored an ultrasonic-assisted method for separating impurity proteins in fish skin. The dissolution amount of impurity proteins, the migration amount of collagen, the fiber structure in fish skin and the subsequent yield and structure of collagen were used to characterize the effect of different ultrasonic pulse intermittent modes on the separation of impurity proteins from fish skin and the effect on fish skin. Collagen fiber structure and influence. The test results show that using ultrasonic pulse mode of 3 s on and 2 s off intermittently (HIU3 s on 2 s off), within 60 minutes, the amount of crude protein dissolved in fish skin is 37.37 mg/g, while the migration of collagen The output is 0.63 mg/g. SEM image observation shows that after HIU3 s on 2 s off pulse treatment, the internal structure of the fish skin is loose and the fish skin collagen fibers are fully stretched, which is conducive to the rapid extraction of subsequent collagen; and the FT-IR image shows that the amide of the fish skin collagen fibers The position of the bond remains unchanged; after subsequent extraction, the yield of collagen is the highest, and its primary structure remains intact. Due to the larger consumption of energy, solvent, and pretreatment period during the conventional separation of crude protein in fish skin, a method of HIU-aided pretreatment of fish skin was provided. In order to reveal the effects of different ultrasonic pulses ways on fish skin and the structure of fish collagen fiber, five following parameters were investigated: total content of proteins removed, loss of collagen during the HIU pretreatment, structure of collagen fiber after the HIU pretreatment, subsequent extraction yield of collagen and SDS-PAGE patterns of collagen . After pretreatment by the way of HIU3 s on 2 s off for 60 min, the content of protein in fish skin removed was 37.37 mg/g without significant loss of collagen (0.63 mg/g). SEM figures showed that, the loose of fish skin and the sufficient extension of collagen fiber were convenient for the subsequent collagen extraction after pretreatment by the way of HIU3 s on 2 s off. FT-IR spectra obtained for fish collagen fiber confirmed the integrated conformation of fish skin collagen. Furthermore, collagen with structural integrity well maintained yielded the highest.
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Original research was done by Liang Jianhua
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