Effects Of Arecoline On Oral Mucosal Fibroblast Lesions
Keywords
Arecoline, Oral Mucosal Fibroblasts, Microfilament Skeleton, Collagen Phagocytosis
Abstract
Objective To analyze the effects of arecoline on microfilament skeleton and collagen phagocytosis of oral mucosal fibroblasts (OMFb). Methods: Normal human oral mucosa was collected and cultured using the tissue block method. Sixth generation cells were taken for intervention with different concentrations of arecoline (0 ¦Ìg/mL, 10 ¦Ìg/mL, 20 ¦Ìg/mL, 30 ¦Ìg/mL, 40 ¦Ìg/mL) and tested for 12 h and 24 h. , cell proliferation after 48 hours; analyze the changes in collagen phagocytosis rate after 24 hours; observe the changes in cell microfilament skeleton after 24 hours. Results After 12 hours of arecoline intervention, there was no statistically significant difference in the proliferation of OMFb with different arecoline concentrations (P>0.05); after 24 hours, the proliferation rate of OMFb increased in the 10 ¦Ìg/mL and 20 ¦Ìg/mL concentration groups, and the proliferation rate in the 20 ¦Ìg/mL concentration group There was no proliferation of OMFb in the >10 ¦Ìg/mL concentration group; there was no proliferation of OMFb in the 30 ¦Ìg/mL and 40 ¦Ìg/mL concentration groups; after 48 hours, all concentrations of arecoline had an inhibitory effect on OMFb proliferation. The collagen phagocytosis ability of OMFb was significantly improved in the 10 ¦Ìg/mL and 20 ¦Ìg/mL concentration groups, while the collagen phagocytosis ability was inhibited in the 30 ¦Ìg/mL and 40 ¦Ìg/mL concentration groups. In addition, the OMFb cell microfilament skeleton in the 10 ¦Ìg/mL and 20 ¦Ìg/mL concentration groups showed a thick bundle-like arrangement, tension fibers appeared, and cell polarity was enhanced, and the changes in the 10 ¦Ìg/mL concentration group were higher than those in the 20 ¦Ìg/mL concentration group; The microfilament skeleton of OMFb cells in the 40 ¦Ìg/mL concentration group was significantly reduced, followed by the 30 ¦Ìg/mL concentration group, and a large number of stained aggregates appeared in the cell cortex. Conclusion Low concentrations of arecoline have a significant promoting effect in enhancing OMFb activity and collagen phagocytosis ability, but high concentrations have inhibitory effects. In addition, low concentrations of arecoline can also enhance the polymerization of microfilament skeleton of OMFb cells, while high concentrations of arecoline can inhibit the polymerization of microfilament skeleton of OMFb cells, which may be one of the main pathogenesis mechanisms of oral submucosal fibrosis.
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Original research was done by Nie Haiyan
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