Effects Of Asymmetric Traction On Type Ii Collagen Of Condylar Cartilage In Adult Rats
Keywords
Traction, Condyle, Type Ii Collagen
Abstract
Objective To understand the effect of extra-articular mechanical stress on the activity of type II collagen synthesis by condylar chondrocytes by observing the changes in type II collagen in the condylar cartilage of adult SD rats caused by asymmetric traction. Methods: 220 10-week-old male SD rats were selected (randomly divided into 104 light force group, 104 gravity group, and 12 control group). The animals in the experimental group used external joint fixation and force device, applying 0.39N and 1.18N respectively. (Light force group and gravity group), the afterburner device will be removed on the 28th day of afterburner. Specimens were obtained at 3, 7, 14, and 28 days after intensification and at 3, 7, 14, and 28 days after stopping traction for immunohistochemical staining. Results The positive signal of type ¢ò collagen was mainly expressed in the cytoplasm of chondrocytes, and was obviously expressed in mature zone and hypertrophic zone cells. At different stages of the experiment, different expression intensities occurred in different parts. After traction force loading, the change in type II collagen expression in the gravity group was not as obvious as that in the light force group, but the changes in the corresponding applied and non-forced sides of the two groups were similar – the applied force side was in the early stage of force application. The expression was down-regulated, and the expression increased after the traction force was removed, and then dropped to the lowest level during the afterburner stage, and then gradually increased; the expression on the non-afterburner side was up-regulated after the afterburner, and the 2 groups reached a peak on the 14th day. . Upon removal of traction, a downregulation occurs, and then both rise again. Conclusion The condylar cartilage of adult individuals still exhibits changes in type II collagen synthesis after being subjected to external forces. The expression of type II collagen appears in the mature zone and hypertrophic zone where protein synthesis is active. Unilateral forward traction has an inhibitory effect on the synthesis of cartilage matrix on the applied side. A larger traction force has a stronger inhibitory effect than a lighter traction force; non-augmented force The nature of the stress experienced by the force side is different, and extracellular matrix synthesis is accelerated.
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Original research was done by Wu Tuojiang, Xu Yue, Li Huang, Qu Lingli, Chen Yangxi
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