Effects Of Basic Fibroblast Growth Factor And Collagen On The Construction Of Tissue Engineered Cartilage In Vitro
Keywords
Bfgf, Collagen, Cartilage, Construction
Abstract
Objective: To explore the effects of bFGF and collagen on the in vitro construction of tissue engineered cartilage using three-dimensional mass culture as the culture system. Methods: Rabbit growth plate chondrocytes were cultured in groups, and bFGF, collagen and combined groups were established. HE staining was used to observe the morphology of new tissue; immunohistochemistry was used to detect the expression of type I and II collagen to observe cell phenotype; Hoechst 33258 method was used to detect cell DNA content; the hydroxyproline method and acian blue method were used to determine the synthesis of collagen and proteoglycans in the matrix. . Results: The histological morphology of new cartilage is similar to natural cartilage; the DNA content of chondrocytes in each experimental group increased significantly; collagen can significantly promote the synthesis of matrix; the expression of type I collagen in each experimental group was less than that of the control group, while the expression of type II collagen was higher. Compared with the control group; the effect of the combined effect group was more obvious. Conclusion: Three-dimensional mass culture can promote matrix synthesis and effectively maintain chondrocyte phenotype; bFGF and collagen are beneficial to the construction of engineered cartilage, and their effects have a synergistic effect. The combined application of the two can further promote cartilage regeneration.
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Original research was done by Wu Fenglin, Yu Li
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