Effects Of Co-Culture Of Keratinocytes And Fibroblasts On Cell Proliferation And Collagen Synthesis Under Pressure
Keywords
Keratinocytes,Fibroblasts,Cell,Proliferation,Collagen,Pressure
Abstract
Objective To study the effect of three-dimensional co-culture of human keratinocytes (HKC) and human fibroblasts (HFB) under pressure on cell proliferation and collagen synthesis. Methods: HKC and HFB were inoculated on chitosan-gelatin scaffolds for 2 days respectively, and then the HKC-chitosan-gelatin complex and HFB-chitosan-gelatin complex after induction differentiation at the air-liquid interface for 1 day were co-cultured for 12 days. h, 3.4 kPa gas pressure was loaded for 24 h, and cultured alone with pressure, cultured alone without pressure, or co-cultured without pressure were used as controls. HE staining was used to observe the distribution and growth of cells in the scaffold, MTT method was used to measure cell proliferation, and a hydroxyproline kit was used to measure the collagen content in the supernatant. Results HE staining showed that both HKC and HFB could proliferate normally into sheets on the chitosan gelatin scaffold; 3.4 kPa pressure or co-culture could promote HKC proliferation and collagen synthesis, and inhibit HFB proliferation and collagen synthesis. Conclusion Pressure and co-culture are important factors affecting the proliferation and collagen synthesis of HKC and HFB. The research results provide a reference for the possible mechanism of transplanting tissue-engineered epidermis combined with pressure therapy to treat hypertrophic scars after surgical scar removal. Objective To investigate effects of 3D co-culture of human keratinocytes (HKC) and human fibroblasts (HFB) under pressure on cell proliferation and collagen synthesis. Methods The HKC and HFB were planted on chitosan-gelatin scaffolds, respectively, for 2 d. The HKC-chitosan-gelatin complex (3D HKC) was cultured at air-liquid interface for 1 d to induce differentiation, and then co-cultured with the HFB-chitosan-gelatin complex (3D HFB) for 12 h. 3.4 kPa pressure was applied on the co-culture group for 24 h. The group of single culture with pressure, the group of single culture without pressure and the group of co-culture without pressure were used as control. HE staining was used to observe distribution and growth of HKC and HFB on chitosan-gelatin scaffolds. MTT method was used to test proliferation of HKC and HFB. Hydroxyproline kit was used to observe collagen concentration of the supernatant fluids. Results HE staining showed that HKC and HFB could grow confluently on chitosan-gelatin scaffolds; 3.4 kPa pressure or co-culture both could promote the HKC proliferation and collagen synthesis, while restraining the HFB proliferation and collagen synthesis. Conclusions Pressure and co-culture play an important role in HKC and HFB proliferation and collagen synthesis. This research finding provides some reference for exploring the therapeutic mechanism of hyperplastic scar from clinical operation of resecting scar by transplanting tissue-engineered skin to the wound and then combined with pressure treatment.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Liu Yang, An Meiwen, Lu Ying, Chen Lingfeng, Ru Mengjie
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
