Effects Of Erchen Decoction With Flavored Erchen Decoction On Extracellular Matrix Remodeling Of Bronchiolar Walls In Chronic Obstructive Pulmonary Disease
Keywords
Chronic Obstructive Pulmonary Disease (Copd) Erchen Decoction Type I Collagen Type Iii Collagen Extracellular Matrix (Ecm) Matrix Metalloproteinase (Mmp)
Abstract
Objective: To observe the effect and mechanism of modified Erchen Decoction on the structural remodeling of bronchioles in chronic obstructive pulmonary disease (COPD) model rats. Methods: 50 SD rats were randomly divided into normal group, model group, Erchen Decoction flavored low, medium and high dose groups (5, 10, 20 g¡¤kg-1), a total of 5 groups, 10 rats in each group. Rat COPD model was prepared by adding cigarette smoke and endotoxin lipopolysaccharide (LPS). After the model was successfully established, each observation group was administered intragastric administration to detect lung function, and real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect matrix metalloproteinases (MMP-1) and MMP in lung tissue. -9 and tissue inhibitor of metalloproteinase (TIMP-1) mRNA expression, immunohistochemistry (IHC) detection of MMP-1, MMP-9, TIMP-1 and type ¢ñ and type ¢ó collagen Expression in bronchioles. Results: Compared with the normal group, the expression of MMP-1, MMP-9, and TIMP-1 mRNA in the lung tissue homogenate of the model group were significantly enhanced (P<0.01). The expressions of type ¢ñ and type ¢ó collagen were significantly enhanced (P<0.05). Compared with the model group, the expression of MMP-1, MMP-9, and TIMP-1 mRNA in the lung tissue homogenate of the medium- and high-dose groups was significantly weakened (P<0.01), and the expression of type ¢ñ and type ¢ó collagen in the bronchiolar wall of the medium- and high-dose groups was significantly reduced (P<0.01). The expression was significantly weakened (P<0.05). Conclusion: The flavored Erchen Decoction has an inhibitory effect on collagen in the extracellular matrix (ECM) of the bronchiolar wall. The mechanism may be related to inhibiting MMP-1, MMP-9, and coordinated inhibition of the expression of TIMP-1 to prevent the deposition of bronchiolar ECM collagen. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Bao Yongsheng, Wu Ke, Ji Shu, Shang Lizhi, Zhang Jing, Wang Junyue, Xie Wenying
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