Effects Of Linear Alkylbenzenesulfonate On Oxidative Stress And Collagen Fiber In Skin Tissue Of Mice
Keywords
Linear Alkylbenzenesulfonate,Mice,Skin Tissue,Oxidative Stress,Collagen Fiber, Linear Alkylbenzenesulfonate,Mice,Skin Tissue,Oxidative Stress,Collagen Fiber
Abstract
Objective: To observe the effects of linear alkylbenzenesulfonate (LAS) on oxidative stress and collagen fibers in mouse skin tissue, and to preliminarily explore the relationship between the two. Methods: 40 healthy Kunming male mice were randomly divided into 4 groups, namely normal control group, LAS low-dose group (150 mg/L), medium-dose group (300 mg/L) and high-dose group (600 mg/L). L), 10 per group. Distilled water and different doses of LAS were applied to the shaved back areas of mice in each group, once a day for 60 days. Observe the general conditions such as diet, daily activities and changes in body weight of the mice in each group; take the skin at the application site for HE and Masson staining to observe the changes in skin tissue structure and collagen fibers; measure the content of hydroxyproline (Hyp) in the skin tissue The content of collagen is used to evaluate the changes in the amount of collagen; the activity of superoxide dismutase (SOD), malondialdehyde (MDA) content in skin homogenates and serum, and the activity of lactate dehydrogenase (LDH) in serum are detected to evaluate changes in oxidative stress. Results: During the experiment, mice in each dose group of LAS had no significant differences from the normal control group in terms of diet, daily activities and mental state; after 4 weeks, the body weight of mice in each dose group was significantly lower than that of the normal control group, and the difference was statistically significant. significance (P<0.01). HE and Masson staining showed that the skin of mice in each dose group of LAS became significantly thinner, epidermal cells were partially necrotic and shed, and there were infiltration of inflammatory cells in the superficial dermis; collagen fibers were reduced, and the fiber arrangement was disordered, loose, and blurred. Various biochemical indicators showed that compared with the normal control group, the MDA content and LDH activity of mice in each dose group of LAS were significantly increased (P<0.01), while the SOD activity and Hyp content were significantly decreased (P<0.01). Conclusion: LAS can induce oxidative stress in mouse skin tissue, destroy the integrity of the skin structure, causing obvious pathological changes, and change the morphology of collagen fibers and significantly reduce their content. Oxidative damage caused by oxidative stress may be the main cause of collagen changes For further information of this article and research, feel free to contact our team for asssitance. Original research was done by ZHAO Wenhong, WANG Jinhua, WANG Kailei, ZHANG Wen
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