Effects Of Liver X Receptor Agonist T0901317 On The Proliferation, Migration And Collagen Formation Function Of Human Embryonic Lung Fibroblasts
Keywords
Liver X Receptor, Fibroblast, Transforming Growth Factor Beta 1, Basic Fibroblast Growth Factor
Abstract
Objective To explore the effect of liver X receptor agonist T0901317 on the proliferation, migration and collagen secretion function of human embryonic lung fibroblasts. Methods Human embryonic lung fibroblasts were cultured and divided into control group, T0901317 group, growth factor group, and growth factor + T0901317 group. The cells in the control group were only cultured with serum-depleted Dulbecco’s modified Eagle culture medium, the T0901317 group was added with 1.00 ¦Ìmol/L T0901317 intervention, and the growth factor + T0901317 group was incubated with 0.25, 0.50, and 1.00 ¦Ìmol/L T0901317 for 2 h respectively. Recombinant human basic fibroblast growth factor and recombinant human transforming growth factor ¦Â1 were added together with the growth factor group and cultured for 24 h. Cell counting kit 8 was used to detect cell proliferation, Transwell method was used to detect cell migration, and hydroxyproline assay was used to detect the ability of cells to secrete collagen. Results Compared with the control group, the proliferation, migration and collagen secretion abilities of cells in the T0901317 group had no significant changes, while the proliferation, migration and collagen secretion abilities of cells in the growth factor group were significantly enhanced. T0901317 can inhibit growth factor-induced fibroblast proliferation, migration and collagen secretion. The greater the concentration of T0901317, the more obvious the inhibitory effect. Conclusion T0901317 may inhibit the proliferation, migration and collagen synthesis of human embryonic lung fibroblasts by inhibiting growth factor function.
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Original research was done by Tan Yan, Gu Wei
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