Effects Of Paeoniflorin On Glucocorticoid Receptors In Peripheral Blood Mononuclear Cells Of Rats With Collagen-Induced Arthritis
Keywords
Paeoniflorin, Collagen-Induced Arthritis, Glucocorticoid Receptor, Reverse Transcription-Polymerase Chain Reaction
Abstract
To study the effect of paeoniflorin (PF) on the glucocorticoid receptor (GCR) of peripheral blood mononuclear cells (PBMCs) in rats with collagen-induced arthritis. The collagen-induced arthritis (CIA) rat model was used. On the 14th day after the initial immunization, PF25, 50, 100mg¡¤kg-1 or triptolide 20¦Ìg¡¤kg-1 or PF50mg¡¤kg-1+RU48615mg¡¤ were administered intragastrically. kg-1, once a day, administered continuously for 28 days. On the 2nd day after the last dose, all experimental rats except the PF+RU486 group had their eyeballs removed to collect blood. They were sacrificed and unimmunized joints were collected from all rats. The expression of GCR¦Á and GCR¦Â in PBMCs was detected by ELISA and reverse transcription-polymerase chain reaction. The expression of GCR¦Á and GCR¦Â mRNA in PBMCs was analyzed by (RT-PCR) method, and the expression of IL-1¦Â, NF-¦ÊBp65, TNF-¦Á, and PGE2 in joint synovial tissue was analyzed by immunohistochemistry. PF50, 100mg¡¤kg-1 can inhibit the expression of IL-1¦Â, NF-¦ÊBp65, TNF-¦Á, and PGE2 in the synovial tissue of CIA rats (P<0.05, P<0.01), while RU48615mg¡¤kg-1 can significantly affect This effect of PF50mg¡¤kg-1 (P<0.05); PF50 and 100mg¡¤kg-1 can upregulate the expression of GCR¦Á and GCR¦ÁmRNA, and downregulate the expression of GCR¦Â and GCR¦ÂmRNA (P<0.01). PF inhibits the joint synovium of CIA rats. Inflammatory mediators may be related to their regulation of GCR expression. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Yi Jianfeng
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