Effects Of Polycaprolactone/Collagen Scaffold Nanofiber Orientation On Morphology, Proliferation And Differentiation Of Mouse Osteoblast Precursor Cells

Keywords

Polycaprolactone/Collagen, Nanofibers, Mct-E Cells, Orientation

Abstract

Objective To explore the effect of polycaprolactone/collagen (PCL/COL) scaffold nanofiber orientation on the morphology, proliferation and differentiation of mouse osteoblast precursor cells (MC3T3-E1). Methods The morphology of non-oriented and oriented PCL/COL nanofiber scaffolds was observed through scanning electron microscopy (SEM); MC3T3-E1 cells were seeded on non-oriented and oriented PCL/COL nanofiber scaffolds, and the morphology was observed through SEM and laser. Confocal microscopy (LSCM) was used to observe the growth morphology of cells on two sets of materials, CCK-8 method was used to detect the proliferation of cells on two sets of materials, and Real-timeRT-PCR was used to detect the functionality of osteogenic differentiation of cells on two sets of materials. Expression of genes type ¢ñ collagen (COL ¢ñ), alkaline phosphatase (ALP), and osteocalcin (OCN). Results The fiber direction of the non-oriented PCL/COL nanofiber scaffold was irregular, while the fiber direction of the oriented PCL/COL nanofiber scaffold was the same. There was no significant difference in fiber diameter between the two (P>0.05), which were (586¡À239) nm respectively. and (625¡À155) nm; SEM and LSCM detection results show that MC3T3-E1 cells grow in a star shape on non-oriented nanofibers and the cytoskeleton has an irregular shape, while on oriented nanofibers they grow in a flat and elongated shape, and The cytoskeleton is neatly arranged along the direction of the nanofibers; CCK-8 detection results show that there is no statistically significant difference in the proliferation rate of MC3T3-E1 cells on non-oriented and oriented PCL/COL nanofiber scaffolds at 1, 3, 5 and 7 days (P> 0.05); Real-timeRT-PCR test results showed that there was no statistically significant difference in the COL¢ñ expression levels of MC3T3-E1 cells on the two groups of materials (P>0.05), while the cells in the non-oriented group expressed more ALP than the oriented group. and OCN, the differences were statistically significant (P<0.05). Conclusion Both non-oriented and oriented PCL/COL nanofiber scaffolds can provide a good microenvironment, which is conducive to the growth and proliferation of MC3T3-E1 cells. At the same time, non-oriented PCL/COL nanofiber scaffolds are more effective than oriented PCL/COL nanofiber scaffolds. It is beneficial to the osteogenic differentiation of MC3T3-E1 cells and is a promising bone tissue engineering scaffold material. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Lu Ju  

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