Effects Of Pulsed Dye Laser On The Procollagen Mrna Expression And Proliferation Of Cultured Fibroblasts In Vitro
Keywords
Smads 585, Nm Pulsed Dye Laser, Fibroblasts, Proliferation, Procollagen Gene
Abstract
Normal skin fibroblasts cultured in vitro were irradiated with pulsed dye lasers of different energy densities, and cultured for 24 hours after irradiation. Their proliferation function was detected by MTT method, and different types of procollagen in their cells were detected by quantitative PCR (LightCycler) method. Expression of genes and SMADs mRNA. The results show that pulsed dye laser with energy density of 5 J/cm2 and 7 J/cm2 can inhibit the proliferation function of normal skin fibroblasts cultured in vitro (P<0.05). At the energy density of 5 J/cm2 can significantly inhibit the expression of type ¢ñ procollagen gene ¦Á1 (P£¼0.01) and SMAD 2, 3, 4, 7 mRNA (P£¼0.05 or P£¼0.01), and downregulate type ¢ñ procollagen gene ¦Á1 and type ¢ó The ratio of procollagen gene mRNA. When the energy density is 6,7 J/cm2, it can inhibit the expression of type III procollagen gene ¦Á1mRNA (P<0.05), but the expression of type I procollagen gene ¦Á1 and ¦Á2 is not significantly different from the control. Sexual differences. It is suggested that the 585 nm wavelength pulsed dye laser has a direct effect on the proliferation of fibroblasts and the expression of some genes related to collagen production, and is related to the energy density. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by YU Hai-yan,FANG Qing,CHEN Da-fang,CHENG Hao, Yu Haiyan, Fang Qing, Chen Dafang, Cheng Hao
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