Effects Of Sodium Ferulate On Acetaldehyde-Induced Hepatic Stellate Cell Proliferation, Activation And Collagen Synthesis
Keywords
Sodium Ferulate Hepatic Stellate Cells (Hsc-T6) Acetaldehyde Cell Proliferation Activated Collagen
Abstract
Objective: To study the effect and mechanism of sodium ferulate (SF) on the proliferation, activation and collagen synthesis of rat hepatic stellate cells (HSC-T6) induced by acetaldehyde in vitro. Methods: HSC-T6 cells were cultured in vitro, and an acetaldehyde-induced HSC-T6 proliferation model was established. A blank group, acetaldehyde group, colchicine group (2.5 ¦Ìmol¡¤L-1) and SF group (1, 10, 25, 50, 100, 200, 400 ¦Ìmol¡¤L-1), detect cell proliferation through cell proliferation and toxicity test (MTS) colorimetric method, and screen the appropriate SF concentration. Western blot was used to detect the expression of ¦Á-smooth muscle actin (¦Á-SMA) in HSC-T6 cells induced by acetaldehyde by SF (400, 200, 100 ¦Ìmol¡¤L-1); enzyme-linked immunosorbent assay (ELISA) detects the concentration of type ¢ñ and type ¢ó collagen, acid hydrolysis method detects hydroxyproline (Hyp) content, real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) detects transforming growth factor-¦Â1/(TGF-¦Â1 /), signal transduction protein Smad2, Smad3, Smad4, Smad7, matrix metalloproteinase-1 (MMP-1) and metalloproteinase inhibitor-1 (TIMP-1) gene mRNA expression changes. Results: Compared with the blank group, 200 ¦Ìmol¡¤L-1 acetaldehyde could significantly induce the proliferation of HSC-T6 in vitro (P<0.01); compared with the blank group, the proliferation of HSC-T6 in the model group significantly increased (P<0.01), ¦Á -The contents of SMA and type I, type III collagen and Hyp were significantly increased (P<0.01), the expression of TGF-¦Â1/, Smad2, Smad3, Smad4, Smad7 mRNA was significantly increased (P<0.01), MMP-1 and TIMP-1 The mRNA expression was significantly increased (P<0.01). Compared with the model group, SF at concentrations of 400, 200, and 100 ¦Ìmol¡¤L-1 could significantly inhibit acetaldehyde-induced HSC-T6 proliferation (P<0.05, P<0.01), significantly reduce ¦Á-SMA and type I and type III Collagen and Hyp content (P<0.01), down-regulated the expression of TGF-¦Â1/, Smad2, Smad3, Smad4 gene mRNA (P<0.05, P<0.01), up-regulated the Smad7 gene mRNA expression (P<0.01), and significantly up-regulated MMP -1 mRNA expression (P<0.05, P<0.01) and down-regulated TIMP-1 mRNA expression (P<0.01). Conclusion: SF can inhibit the proliferation, activation and collagen secretion of HSC-T6 cells induced by acetaldehyde in vitro by regulating the TGF-¦Â1/Smad and MMP-1/TIMP-1 signaling pathways. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Feng Guohua, Zhang Zhibi, Xu Zhi, Miao Zi'e, Cai Ting
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