Effects Of The Microstructure Of Two Mineralized Collagens On The Morphology Of Osteoblast-Like Cells Mg63
Keywords
Minerals, Collagen, Tissue Scaffolds, Cell Adhesion, Bone Regeneration
Abstract
Objective: To study the effect of the microstructure of two types of mineralized collagen on the adhesion and morphology of human osteoblast-like cells mg63. Methods: Traditional mineralization method and biomimetic mineralization method were used to prepare extrafibrillarly-mineralized collagen (emc) and intrafibrillarly-mineralized collagen (imc) scaffold materials respectively. Human osteoblast-like cells mg63 were used as Cells were used in the experiment, and scanning electron microscopy (SEM) was used to detect the microstructure of the two scaffold materials and the interaction between cells and materials. Laserscanningmicroscope (lsm) was used to detect the adhesion and morphology of cells seeded on the material. Results: Different mineralization modes affected the microstructure of collagen. In the emc group, cluster-like crystals can be seen randomly deposited on the surface of the nanofiber bundle, while in the imc group, the surface of the fiber bundle is smooth and there is no crystal deposition. Energy spectrum analysis results show that it contains crystals inside, which are carbon-containing calcium-deficient hydroxyapatite, which is similar to the composition of natural bone mineralizing collagen. LSM results show that imc can promote mg63 cell stretching more than emc scaffold material. Fluorescence quantitative analysis results showed that the adhesion force of mg63 cells to imc scaffold material (18.54¡À2.71) was significantly higher than that of emc scaffold material (14.29¡À1.32). SEM results show that both mineralized collagens have good biocompatibility, and cells show different morphologies on different scaffolds. Conclusion: The microstructure of mineralized collagen affects the morphology of cells, and the imc scaffold material can promote the adhesion and extension of mg63 cells, which will help develop new bionic materials for alveolar bone regeneration.
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Original research was done by Liu Yan, Fu Yu, Liu Shuai, Zhou Yanheng
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