Enzymatic Extraction Of Egg Shell Membrane Collagen
Keywords
Eggshell Membrane, Collagen, Enzymatic Method, Extraction
Abstract
Collagen was extracted from egg membranes using enzymatic hydrolysis. The effects of enzyme type, pH value, enzyme dosage, material-to-water ratio and extraction time on collagen extraction were studied. A single factor test was used combined with L9(34) orthogonal test optimization. It was concluded that the optimal process conditions for enzymatic extraction of collagen from eggshell membranes were enzymatic hydrolysis of 6% papain at room temperature for 5 hours at pH 5 and a material-to-water ratio of 1:100 (g/mL). The extraction rate reaches 0.91%. Obtaining an efficient process for extracting collagen not only solves the environmental pollution problem of discarded egg shell membranes, but also has great practical value in the collagen production industry.
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Original research was done by Ren Meng, Liu Jingbo, Sun Yanbo, Gong Xintong, Zhang Yuanyuan, Yin Jianwei, Zhao Songning
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