Experimental Study On Composite Culture Of Goat Jaw Bone Marrow Mesenchymal Stem Cells And Nanocrystalline Collagen Bone Materials In Vitro Repair Of Rabbits Critical Mandibular Defects With Bmscs/Nhac Complex
Keywords
Bone Marrow Mesenchymal Stem Cells, Nanocrystalline Collagen Bone Material, Tissue Engineering, Bone Marrow Mesenchymal Stem Cells, Nanocrystalline Collagen Bone Material, Tissue Engineering
Abstract
Abstract Purpose: To explore the combination of goat bone marrow mesenchymal stem cells (BMSCs) and nanocrystalline collagen bone material (NHAC) composite culture in vitro, and to preliminarily explore the effect of BMSCs/NHAC complex on rabbit mandibular defect repair in vivo. Methods: The third generation goat BMSCs were co-cultured with NHAC, and MTS was used to detect cell proliferation, alkaline phosphatase activity was used to detect ALP activity, and real-time fluorescence quantitative PCR (qRT-PCR) was used to detect osteogenic genes. A rabbit mandibular defect model was established, and osteogenic differentiation-induced BMSCs/NHAC complex was implanted into the defect. Goldner’s trichrome staining experiment and micro-CT were used to evaluate bone regeneration. Results: On the 5th and 7th days of culture, the A490 values of the co-culture group were significantly higher than those of the normal group (P<0.05). ALP activity in the co-culture induction group was significantly higher than that in the normal induction group (P<0.05). The expression levels of OCN, Runx2, and Osterix mRNA in the co-culture induction group were significantly higher than those in the normal induction group (P<0.05). Compared with the Block group, the bone volume density of the NHAC group and the BMSCs/NHAC group was significantly increased (P<0.05). Compared with the NHAC group, the bone volume density of the BMSCs/NHAC group was significantly increased (P<0.05). Conclusion: NHAC can provide a suitable environment for the adhesion, proliferation and differentiation of BMSCs, and the BMSCs/NHAC complex can promote bone regeneration in rabbit mandibular defects. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Li Dongmei, Liu Xinhui, Li Qingxing, LI Dong-mei, LIU Xin-hui, LI Qing-xing.
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