Expression Of Rankl And M-Csf In Joint Synovium Of Cia Rats And Their Intervention Effects
Keywords
Collagen-Induced Arthritis, Rat, Iguratimod, Methotrexate
Abstract
Objective To preliminarily explore the expression of RANKL and M-CSF in the joint synovium of CIA rats and the intervention effects of T-614 and MTX. Methods Rats were immunized with collagen emulsion mixed with chicken type II collagen and complete Freund’s adjuvant to establish a CIA rat model. CIA rats were randomly divided into model control group, T-614 group, MTX group and T-614+MTX. Group. Rats in the drug intervention group were treated with T-614, MTX and T-614 combined with MTX for 3 weeks respectively. Arthritis index (AI) score was used to evaluate the degree of arthritis, HE staining was used to observe the morphological changes of ankle joint tissue, X-ray films were used to observe bone erosion, high-frequency ultrasound was used to observe ankle joints and measure synovial thickness, and Westernblot method was used to detect synovial RANKL. and M-CSF expression. Results Compared with the normal control group, the AI score, pathology score, X-ray score, membrane thickness under ultrasound, and expression of RANKL and M-CSF in joint synovium all increased in the model control group (P<0.05); compared with the model control group, The above observed indicators in the T-614 group, MTX group, and T-614 combined with MTX group were all reduced (P<0.05); compared with the T-614 group and MTX group, the above observed indicators in the T-614 combined with MTX group were all reduced (P<0.05). 0.05); there was no statistically significant difference in the above observation indicators between the T-614 group and the MTX group (P>0.05). Conclusion RANKL and M-CSF are involved in the occurrence and development of joint synovial lesions in CIA rats. T-614 and MTX may delay the progression of joint synovial lesions by reducing the production of cytokines RANKL and M-CSF. T-614 and MTX has certain synergistic effects.
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Original research was done by Wang Qianqian, Wang Meimei
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