Extraction, Identification And Physical And Chemical Properties Of Collagen From The Skirt Of Chinese Soft-Shell Turtle
Keywords
Chinese Soft-Shell Turtle, Skirt, Collagen, Peptide Fingerprint Mass Spectrometry, Circular Dichroism Spectrum, Thermal Stability
Abstract
Abstract In order to increase the added value of Chinese soft-shell turtle products and develop new collagen resources, this study used the skirt of Chinese soft-shell turtle as raw material and used different extraction methods to extract acid-soluble collagen (ASC) and pepsin-soluble collagen. (pepsin-soluble collagen, PSC), and the structure and physical and chemical properties of the two collagens were analyzed. The structure of collagen was analyzed using scanning electron microscope (SEM) and circular dichroism spectroscopy (CD), and SDS-PAGE gel electrophoresis and peptide fingerprint mass spectrometry (matrix-assisted laser desorption ionization- Time of flight, MALDI-TOF) and amino acid analyzer were used to conduct comparative analysis of proteins and amino acids on ASC and PSC, and differential calorimetry scanning (DSC) was used to conduct thermal stability analysis of collagen. The results show that the yields of ASC and PSC are 26.79% and 37.50% respectively; PSC has a more complete three-level helical structure compared with ASC; ASC and PSC are both type I collagen, and have higher imino acids and sperm. amino acid content; the thermal denaturation temperatures of ASC and PSC are 38.39 and 38.11 ¡æ respectively, which are higher than common pig skin and fish skin collagen, and have better thermal stability. This study provides a theoretical basis for in-depth research and development of skirt resources of Chinese soft-shell turtle
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Original research was done by Zhang Qiang, Wang Hongxin, Fu Anwei, Huang Xin
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