Extraction Process And Parameter Optimization Of Active Collagen From Grass Carp Scales
Keywords
Grass Carp Scales, Collagen, Extraction, Parameter Optimization
Abstract
Using grass carp scales as raw materials, acid-soluble collagen (ASC) and enzyme-soluble collagen (PSC) were extracted under conditions below the protein denaturation temperature, and the pretreatment method of fish scale raw materials and collagen extraction process were optimized. The results show that in the pretreatment process of fish scale raw materials, the optimal process conditions for removing impurity proteins in the raw materials using 0.1mol/L Na2CO3 solution as the reagent are room temperature, material-liquid ratio 1:40 (g/mL), 300r/ min, stir 3 times, 6 hours each time; the optimal process conditions for removing mineral impurities in raw materials using 0.3 mol/L EDTA as reagent are room temperature, material-liquid ratio 1:60, stir 3 times, 12 hours each time; The optimal extraction process conditions for ASC are: extraction temperature not higher than 25¡ãC, solid-liquid ratio 1:60, acetic acid concentration 0.8mol/L, extraction 3 times, 24 hours each time; the optimal extraction process conditions for PSC are: pepsin The dosage is 3.0% of the fish scale raw material mass, acetic acid concentration 0.8mol/L, material-liquid ratio 1:30, extraction temperature 4¡ãC, extraction 2 times, 24h each time
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Original research was done by Liu Liangzhong, Zhang Hanjun, Gui Meng, Wang Haibo, Wang Mengjin, Wang Yan
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