Fluorescent Probe Method To Study The Aggregation State Of Collagen In Aqueous Solution
Keywords
Fluorescent Probe, Collagen, Fluorescence Polarization, Aggregation
Abstract
An intramolecular charge transfer compound, 3-methoxy-4’N,N-dimethylaminoflavonoid (DMMF), was used as a fluorescent probe to study the aggregation state of collagen in aqueous solution. The research results found that when the collagen concentration increased to above 0.5mg/mL, the fluorescence intensity of DMMF at 465nm had a sudden jump; as the concentration continued to increase, the fluorescence intensity became larger and larger. It is proved that collagen has obvious aggregation behavior as the concentration increases in aqueous solution. Fluorescence polarization method was used to measure the fluorescence polarization characteristics of DMMF in this system, and it was found that when the collagen concentration increased to above 0.5 mg/mL, the system had a higher microviscosity, further confirming the aggregation characteristics of collagen in aqueous solution.
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Original research was done by Shi Xiangyang, Wu Shikang, Sun Caomin
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