High Performance Chromatography/Mass Spectrometric Analysis Of The Collagen ¢ñ/¢ó Ratio In Rat Skin At Different Stages Of Growth Liquid Chromatography/Mass Spectrometric Analysis Of The Collagen ¢ñ/¢ó Ratio In Rat Skin At Different Stages Of Growth

Keywords

Collagen,Separation,Enzymatic Degradation,High Performance Liquid Chromatography,Mass Spectrometry Collagen,Content,Enzymatic Degradation,High Performance Liquid Chromatography,Mass Spectrometry

Abstract

The collagen content in the dermis of mammalian skin accounts for about 70%, mainly type I, type III and a small amount of type V collagen. The total collagen in rat skin was extracted using dilute acid dissolution and enzymatic methods, and the collagen was The crude extract was denatured at 60¡ãC and then degraded with trypsin. The characteristic peptides of the two collagens were analyzed by liquid chromatography/mass spectrometry, and the relative contents of type I and type III collagen in the skin of rats at different growth stages were studied. Results It shows that the relative content of type III collagen in rat skin gradually decreases with the extension of the growth period, while the relative content of type I collagen gradually increases, and the ratio of the two collagens tends to be stable after 8 weeks. It shows that the use of high-efficiency liquid It is feasible to analyze collagen types and their dynamic changes in tissues using chromatography/mass spectrometry, providing an experimental basis for better clinical applications.

For further information of this article and research, feel free to contact our team for asssitance.

Original research was done by CUI Lin, ZHANG Gui-feng, LIU Tao, BI Jing-xiu, MA Run-yu, SU Zhi-guo, Cui Lin, Zhang Guifeng, Liu Tao, Guan Jingxiu, Ma Runyu, Su Zhiguo

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.