Hplc Method For Determination Of Ergosterol In Puffball Medicinal Materials Of Different Origins And Varieties
Keywords
Puffball Ergosterol Hplc Quality Evaluation Medicinal Material Quality
Abstract
Objective To establish an HPLC method for quality evaluation of puffball Lasiosphaera seu Calvatia using ergosterol as a measurement index, and conduct a comparative study on the amount of ergosterol in 42 batches of different commercial puffballs. Methods HPLC was used to determine ergosterol in puffballs from multiple origins and varieties. The chromatographic conditions were BDS HYPERSIL C18 column (250 mm×4.6 mm, 5 μm); volume flow rate 1.0 mL/min; detection wavelength 282 nm; The column temperature was 30°C; the injection volume was 20 μL; gradient elution was performed with acetonitrile (A)-0.1% formic acid aqueous solution (B) as the mobile phase: 0 to 15 min, 95% A, 15 to 50 min, 100% A. As a result, the extraction method and HPLC determination method of ergosterol in puffballs were established. The ergosterol content in puffballs of different varieties and origins ranges from 0.009 9% to 0.150 3%. Conclusion There are significant differences in the amount of ergosterol in different commercial puffballs, indicating that the quality of puffball medicinal materials currently available on the market is uneven. Therefore, the quality control of puffball medicinal materials needs further standardization and standardization.
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Originate research is done by Guan Shengjiang, Wu Haiyan, An Fuli, Su Shulan, Zhu Shan, Wang Qiao, Xiang Congkun, Chen Yingchun, Huang Huaipeng DOI: 10.7501/j.issn.0253-2670.2016.08.025
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【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
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【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
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【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
【Specification】： 99.5% Powder
【Package】：10mg
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