In Vitro Three-Dimensional Culture Of Cardiomyocytes Using Collagen Membrane As Scaffold
Keywords
Cardiomyocytes, Collagen, Scaffold, Three-Dimensional, Culture
Abstract
Cardiomyocytes obtained from neonatal rat ventricular myocardium were inoculated on rat tail collagen membrane three-dimensional scaffolds and tissue culture plates. Cell morphology, cell beating, specific glucose consumption rate (qglu), specific lactate production rate (qlac), and lactate conversion rate were measured. The activities of (ylac/glu), creatine kinase and lactate dehydrogenase were used as observation indicators to compare the differences between the three-dimensional (3d) culture of cardiomyocytes in rat tail collagen membrane and the two-dimensional (2d) culture of tissue culture plates. The neonatal rat cardiomyocytes cultured on the rat tail collagen membrane formed intercalary disk junctions on the 5th day, forming a 3-day culture of cardiomyocytes with an area of approximately 80 mm3 and autonomous synchronous contraction visible to the naked eye. The mean values of ª«qglu, qlac and ylac/gluªª of neonatal mouse cardiomyocytes in the 3d culture system were 7.37¦Ìmol/10.6cells/d, 2.92¦Ìmol/10ª¬6cells/d and 0.38¦Ìmol/¦Ìmol respectively; in the 2d culture system, the mean values of ª«qglu, qlac and ylac/gluªª were respectively The mean values of qglu, qlac and ylac/gluªª of cardiomyocytes were 7.59¦Ìmol/10.6cells/d, 3.83¦Ìmol/10.6cells/d and 0.51¦Ìmol/¦Ìmol respectively. There was no significant difference in the activities of creatine kinase and lactate dehydrogenase in neonatal rat cardiomyocytes in the two culture systems. Experimental results show that cardiomyocytes cultured on rat tail collagen membrane maintain the metabolic activity and contractile function of normal cardiomyocytes. ª«ª«
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Original research was done by Liu Xingmao Liu Hongxiong Fuyin Chen Zhaolie*
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