Inhibitory Effect Of Haddock Skin Collagen Tryptic Peptide On Uvb-Induced Photodamage In Hacat
Keywords
Haddock Skin Collagen, Trypsin, Peptides, Molecular Docking, Light-Damaged Hacat Cells
Abstract
Objective: To study the antioxidant and photodamage-inhibiting effects of tryptic hydrolyzate peptides from haddock (Melanogrammus aeglefinus) skin collagen, and to improve the reuse value of fish skin. Methods: Single factor experiment and response surface experimental design were used to explore the optimal enzymatic hydrolysis process of haddock skin collagen. The peptide sequence of the prepared enzymatic peptide was determined by matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF MS/MS). The CDOCKER module in Discovery Studio was used to conduct molecular docking of the obtained advantageous peptides with the antioxidant-related Kelch-like epichlorohydrin-related protein-1 (Keap1). After pretreating human epidermal keratinocytes (HaCaT) with polypeptides of different mass concentrations, medium-wave ultraviolet rays were used to induce HaCaT cells to construct a photodamage model. (glutathione peroxidase, GSH-Px) activity, malondialdehyde (MDA) content, and cell apoptosis and cycle to study the ability of enzymatic peptides to inhibit photodamage. Results: The degree of hydrolysis of haddock skin collagen was 78.33% under the conditions of trypsin mass fraction 1.23%, enzymatic hydrolysis temperature 52.95?¡æ, and enzymatic hydrolysis time 1.75?h. MALDI-TOF MS/MS determined that the dominant peptide sequences in the enzymatic hydrolyzate were V-IFFVTMGTP-R, L-SIVPV-R and L-MHT-T. Through molecular docking, it was found that V-IFFVTMGTP-R binds to Keap1 most stably, thus predicting its antioxidant effect. When the light-damaged HaCaT cells were treated with 250?mg/mL enzymatic solution, their SOD and GSH-Px activities were significantly increased compared with the model group (P<0.01), and the MDA content was extremely significantly reduced (P<0.01). Conclusion: Haddock skin collagen trypsin-enzymatic peptide has antioxidant activity and has a certain inhibitory effect on photodamage. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Si Kaixue, Zhou Jun, Cui Chenqian, Zhang Hongyan, Li Yanyan, Wang Chaoyang, Lu Chenyang, Su Xiurong, Dong Lisa, Han Jiaojiao
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
