Inhibitory Effect Of Madecassoside On Collagen-Induced Arthritis In Mice
Keywords
Abstract
Study the effect of madecassoside (asiaticoside) on collagen-induced arthritis (cia) in mice, and preliminarily explore its mechanism of action. Establish a cia model, detect the swelling degree of mouse paw inflammation, perform pathological examination of joint tissue with paraffin sections and He staining, detect spleen lymphocyte proliferation response with MTT method, detect cyclooxygenase-2 (cyclooxygenase-2, cox-2) protein expression, the eia method was used to measure prostaglandine2 (pge2) levels in joint soft tissues, and the elisa method was used to detect the levels of inflammatory factors tnf-¦Á and il-6 in the serum. Intragastric administration of asiaticoside (10, 20 and 40 mg¡¤kg-1) can dose-dependently reduce the symptoms of arthritis in cia mice, inhibit the lymphocyte proliferation response induced by cii collagen in cia mice, and reduce the incidence of arthritis in cia mice. The high level of cox-2 expression and pge2 content in the soft tissue of the ankle joint reduces the levels of inflammatory factors tnf-¦Á and il-6 in the serum; at the same time, pathological examination found that it can improve local arthritis symptoms and inhibit the proliferation of synovial cells in cia mice. Reduce inflammatory cell infiltration. The results show that asiaticoside has an inhibitory effect on CIA, and its mechanism may be related to inhibiting lymphocyte proliferation, reducing cox-2 expression, and promoting the release of inflammatory factors tnf-¦Á and il-6.
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Original research was done by Li Hongzhong, Wan Jingyuan, Zhang Li, Zhou Qixin, Luo Fuling, Zhang Zhuo
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