Inhibitory Effect Of Sinomenine On Nf-¦ªb In Synovial Cells Of Rats With Collagen-Induced Arthritis
Keywords
Sinomenine, Collagen-Induced Arthritis, Synovial Cells, Nuclear Transcription Factor-¦ªb, Nuclear Translocation
Abstract
Objective To observe the effect of sinomenine (SN) on the DNA binding activity and nuclear translocation of nuclear factor-¦ÊB (NF-¦ÊB) in synovial cells of rats with collagen-induced arthritis (CIA), in order to elucidate this Possible mechanisms of anti-inflammatory drugs. Methods CIA rats were used as models, and synovial cells from knee joints were collected and cultured in vitro. Normal control group, CIA group, CIA+methotrexate 10 ¦Ìmol/L (positive control group), CIA+SN 50 ¦Ìmol/L group, and CIA+SN 500 ¦Ìmol/L were established. Group L. Fluorescent labeling and confocal laser scanning microscopy were used to detect nuclear translocation of NF-¦ÊB p65 subunit in synovial cells, and electrophoretic mobility change assay (EMSA) was used to detect the binding activity of NF-¦ÊB to DNA sequences. Results Compared with the normal control group, obvious nuclear translocation of p65 subunit was seen in CIA rat synoviocytes, and the DNA binding activity of NF-¦ÊB was significantly increased (P<0.01). When SN was at 50 ¦Ìmol/L and 500 ¦Ìmol/L, It can inhibit the nuclear translocation of p65 subunit and the DNA binding activity of NF-¦ÊB in CIA rat synoviocytes in a concentration-dependent manner. Methotrexate can downregulate the DNA binding activity of NF-¦ÊB but does not inhibit p65 at 10 ¦Ìmol/L. Nuclear translocation of subunits. Conclusion SN inhibits NF-¦ÊBp65 nuclear translocation and NF-¦ÊB DNA binding activity in synoviocytes, which is one of its anti-inflammatory mechanisms. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Huang Xiaolu, Hao Fei, Wang Yong, Fang Yongfei
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