Interleukin 1#Beta# Inhibits Collagen Synthesis In Cardiac Fibroblasts And Promotes Its Decomposition

Keywords

Interleukin 1#Beta#, Fibroblasts, Collagen, Matrix Metalloproteinases

Abstract

This article applies ~(3)H–thymidine (~(3)H–thymidine, ~(3)H–TdR) incorporation method and ~(3)H–proline (~( 3)H–proline, ~(3)H–Pro) incorporation method to observe the effect of interleukin–1#beta#, IL–1#beta# on Spague–Dawley milk Effects on DNA and collagen synthesis in mouse cardiac fibroblasts, and using gelatin zymography and Western blot to detect matrix metalloproteinases (MMPs) MMP–2, MMP–9 activities and MMP–2 and MMP–9 For protein expression, RT-PCR was used to detect the mRNA expression of MMP–2 and MMP–9. The results showed that: (1) After different concentrations of IL–1#beta# (0.1, 1, 10, 100 ng/mL) acted on cells for 24 h, the amount of H–TdR incorporated in each group was significantly higher. The control group was lower, and the incorporation amount of ~(3)H–Pro was significantly reduced; while 0.01 ng/mL IL–1#beta# acted on CFs, and the incorporation amount of ~(3)H–TdR was significantly reduced. ~(3) The amount of H–Pro incorporated has no significant effect; (2) Different doses of IL–1#beta# stimulate the activity of MMP–2 and MMP–9 to increase in a dose-dependent manner. IL–1#beta# increases MMP–2 protein expression, respectively (0.01 ng/L: 1.35¡À0.05 times; 0.1 ng/mL: 1.56¡À0.10 times; {sl P}<0.05; 1 ng/m: 2.15¡À0.32 times; 10 ng/mL: 2.34¡À0.19 times; 100 ng/mL: 2.41¡À0.26 times; {sl P}<0.01), while increasing MMP--9 protein expression (0.01 ng/mL: 1.38¡À0.20 times; {sl P}<0.05; 0.1 ng/mL: 2.37¡À0.21 times; 1 ng/mL: 2.56¡À0.31 times; 10 ng/mL: 2.60¡À0.19 times; 100 ng/mL: 2.67¡À0.15 times; { sl P}<0.01); (3) The activities, mRNA and protein expressions of MMP--2 and MMP--9 were all higher than those in the control group. IL--1#beta# stimulates MMP--2 mRNA expression to increase (0.01 ng/mL: 1.81¡À0.16 times; {sl P}<0.5; 0.1 ng/mL: 2.17¡À0.19 times; 1 ng/mL: 2.18 ¡À0.04 times; 10 ng/mL: 2.27¡À0.06 times; 100 ng/mL: 2.31¡À0.07 times; {sl P}<0.01) and increased MMP--9 mRNA expression (0.01 ng/mL: 1.36¡À0.13 times ; {sl P}<0.05; 0.1 ng/mL: 1.49¡À0.08 times; 1 ng/mL: 1.50¡À0.11 times; 10 ng/mL: 1.51¡À0.24 times; 100 ng/mL: 1.52¡À0.31 times; {sl P}<0.01). The above results show that IL--1#beta# reduces the synthesis of collagen by reducing the cell division of cardiac fibroblasts, and at the same time promotes the transcription and post-transcriptional expression of MMP--2 and MMP--9 to promote the decomposition of collagen. It is suggested that it plays a certain role in the process of myocardial remodeling. For further information of this article and research, feel free to contact our team for asssitance. Original research was done by Ji Aimin, Song Xudong, Li Zhiliang, Xiao Hua, Sudan, Chen Aihua  

About ETChem

ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.

ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.

As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.