New Online Purification Pre-Treatment Hplc-Ms/Ms For Determination Of Ergosterol And Ergosterone In Flavors And Fragrances
Keywords
Ergosterol, Ergosterone, Online Purification Pre-Treatment, High Performance Liquid Chromatography-Tandem Mass Spectrometry, Flavors And Fragrances
Abstract
new online purification pre-treatment device was established to determine ergosterol and ergosterone in flavors and fragrances combined with high performance liquid chromatography-tandem with mass spectrometry (HPLC-MS/MS). The pretreatment device combines matrix dispersion extraction and column chromatography to extract and purify the sample, and then concentrate the sample and analyze it using HPLC-MS/MS. The HPLC-MS/MS method uses methanol and water as mobile phases, gradient elution at a flow rate of 0.4?mL/min, liquid chromatography separation using a C18 chromatographic column, atmospheric pressure positive ion mode ionization, multiple reaction monitoring mode detection, and internal standard method for quantitative analysis. The results show that this method has convenient pre-treatment and strong stability, the solvent can be recovered, purification, extraction and concentration are integrated; the target substances ergosterol and ergosterone can be separated and detected within 5 minutes, in the range of 1.5 to 250 μg/mL It has good linearity, the linear correlation coefficient of each test substance is greater than 0.999, the detection limit is 0.32 ~ 0.35μg/mL, under different addition levels, the average recovery rate is 86% ~ 96%, compared with the standard The deviation is between 3.1% and 3.6%. This method can be well applied to the determination of ergosterol and ergosterone in actual samples of flavors and fragrances.
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Originate research is done by Ni Chaomin, Kong Weisong, Li Jing, Li Xuemei, Yang Guangyu, Yang Yekun, Wang Xiaohui, Mi Qili, Chen Jianhua
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Ergothionine (L-(+)-Ergothioneine, EGT) represents the sole natural 2-thioimidazole amino acid. This compound possesses the unique ability to permeate cell mitochondria, directly engaging in antioxidant activity at the cellular core. Furthermore, its application extends across various medical procedures, including organ transplantation, cellular preservation, and ophthalmic surgery, underscoring its significant value to human health.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
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L-(+)-Ergothioneine (Food grade)
Ergothionine (L-(+)-Ergothioneine, EGT) is distinguished as the exclusive natural 2-thioimidazole amino acid. Notably, EGT exhibits an absorption wavelength akin to DNA within the ultraviolet (UV) spectrum, effectively safeguarding against UV-induced damage. Moreover, EGT’s chemical stability allows for its accumulation in the skin cells, offering protection without toxicity.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
【Maximum Levels】：For the general population (excluding pregnant and lactating women), a daily intake of 30 mg is recommended. For children over the age of 3 years, a daily dosage of 20 mg is advised.
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Ergothionine (L-(+)-Ergothioneine, EGT) is the sole natural 2-thioimidazole amino acid. EGT possesses an absorption wavelength comparable to that of DNA within the ultraviolet (UV) absorption spectrum, enabling effective protection against UV harm. Additionally, the chemical stability of EGT facilitates its accumulation in the abdominal skin cells, while maintaining non-toxic characteristics.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【INCI】：ERGOTHIONE INE
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Ergothionine (L-(+)-Ergothioneine, EGT) stands as the exclusive natural 2-thioimidazole amino acid. It features an absorption wavelength akin to DNA within the ultraviolet (UV) spectrum, thereby offering effective defense against UV-induced damage. Moreover, EGT exhibits potent antioxidant properties, capable of suppressing the genesis of diverse oxidative active agents. Its broad application spans functional skincare products, functional foods, organ transplantation, cellular preservation, and eye surgery, among other therapeutic procedures.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
【Molecular Weight】：229.2993
【Specification】： 99.5% Powder
【Package】：10mg
【Function】：Calibration and validation of instrument functionality, examination, authentication, and quantification of content.
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Ergothionine (L-(+)-Ergothioneine, EGT) is uniquely identified as the sole natural 2-thioimidazole amino acid. Possessing an absorption wavelength comparable to DNA within the ultraviolet (UV) spectrum, EGT is adept at providing effective protection against UV degradation. Additionally, EGT is characterized by a robust antioxidant capability, inhibiting the development of a wide range of oxidative active compounds. This has led to its extensive application in functional skincare products, functional foods, organ transplantation, cell preservation, ocular surgery, and various other therapeutic practices.
【CAS No.】:497-30-3
【English Name】:L-Ergothioneine
【Formula】：C9H15N3O2S
【Specification】： 99.5% Powder
【Package】：10mg
【Function】：Bioassay, content or potency determination of antibiotics or biochemical drugs.
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