Optimization Of Extraction Process Conditions For Sika Deer Tendon Collagen
Keywords
Deer Tendon, Collagen, Pepsin, High Performance Gel Chromatography
Abstract
In order to extract and prepare collagen from deer tendon using pepsin enzymatic hydrolysis method. Single factor test and orthogonal test were used to screen out the optimal conditions for extracting deer tendon collagen: acetic acid volume fraction 5.5%, solid-liquid ratio 1:7 (mL/g), extraction time 14h, pepsin to substrate ratio 1 :1000(g/g), enzymatic hydrolysis time 24h. The verification experiment results showed that the yield of deer tendon collagen extracted by this method was 74.56%, and the protein content was 65.54%. This experiment optimized the extraction process of sika deer tendon collagen, which is simple and has high extraction rate.
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Original research was done by Zhang He, Xu Yunfeng, Li Yuntong, Zhao Daqing, Zhao Yu
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