Optimization Of Extraction Technology For Enzymatic Hydrolysis Of Collagen From Antler Base Of ª«Cervus Elaphus Using Response Surface Methodology
Keywords
Response Surface Methodology,Antler Base Of Cervus Elaphus,Collagen,Optimization Of Extraction Technology,Pepsin
Abstract
¡¾Objective¡¿The response surface method was used to optimize the hydrolysis and extraction process of deer antler disk collagen. [Method] Using red deer antler disks as raw materials and collagen extraction rate as an indicator, five factors including material-to-liquid ratio, pH value, enzyme amount, enzymatic hydrolysis temperature, and enzymatic hydrolysis time were selected to conduct a single-factor experiment to determine the optimal value of each factor. Excellent extraction conditions. On the basis of the single factor experiment, pH value, enzyme amount, enzymatic hydrolysis temperature, and enzymatic hydrolysis time were selected as influencing factors, and a 4-factor and 3-level Box-Behnken experimental design was conducted. The response surface method was used to analyze the effects of the four factors on collagen. Effect of extraction rate. [Results] The results of the single factor test showed that the collagen extraction rate was the highest when the material-to-liquid ratio was 1:20, the pH value was 1.8, the enzyme amount was 4%, the enzymatic hydrolysis temperature was 37 ¡ãC, and the enzymatic hydrolysis time was 6 h. The optimal extraction process obtained by the response surface method is: pH 1.77, enzyme amount 3.94%, enzymatic hydrolysis temperature 36.78 ¡æ, enzymatic hydrolysis time 5.39 h. Taking into account the actual situation, the optimal extraction process for red deer antler disk collagen predicted by the model The extraction process was revised. The revised process was as follows: pH 1.8, enzyme amount 4%, enzymatic hydrolysis temperature 37¡ãC, and enzymatic hydrolysis time 5 h. Under these conditions, the extraction rate of collagen from red deer antler discs reached 83.32%. The order of importance of the impact of the four factors on the collagen extraction rate is: enzymatic hydrolysis temperature > enzyme amount > pH value > enzymatic hydrolysis time. [Conclusion] A quadratic polynomial model for enzymatic extraction of deer antler disk collagen was established, and the optimal process parameters with high collagen extraction rate were obtained, which effectively shortened the collagen extraction time. ¡¾Objective¡¿Extracting technology of collagen from antler base of Cervus elaphus was optimized using response surface methodology.¡¾Method¡¿Taking antler base of Cervus elaphus as material and collagen extraction rate as index,the effects of material-liquid rate,pH,enzyme loading ,hydrolysis temperature and hydrolysis time on collagen yield were investigated by single factor experiments and the optimal condition of each factor was determined.Then, a four-factor and three-level Box-Behnken design coupled with response surface analysis was carried out to investigate the effects of pH value,enzyme loading,hydrolysis temperature,and hydrolysis time on collagen extraction rate.¡¾Result¡¿Single factor experiments showed that the highest extraction yield was obtained when material liquid rate,pH,enzyme loading,hydrolysis temperature and hydrolysis time were 1 ¡Ã20,1.8,4%,37 ¡æ and 6 h,respectively.The optimal condition pH,enzyme loading,hydrolysis temperature and hydrolysis time were 1.77,3.94%,36.78 ¡æ and 5.39 h determined by response surface methodology.Considering the actual situations , the obtained optimal extracting methods for collagen production from antler base had pH, enzyme loading, hydrolysis temperature and hydrolysis time of 1.8, 4%, 37 ¡æ, and 5 h. Under the optimal conditions, collagen extraction rate from antler base was 83.32% .The importance of four factors to the extraction of collagen was in the order of hydrolysis temperature>enzyme loading>pH value>hydrolysis time.¡¾Conclusion¡¿The extraction technology of antler collagen with pepsin enzymatic hydrolysis was optimized.A quadratic regression equation model was established and the optimum procedure parameters of enzymatic hydrolysis of collagen were obtained, which effectively shortened extraction time
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Wei Gongqing, Liu Shaohua, Fan, Ning, et al.
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.
