Osteogenesis Of Sirna-Loaded Collagen/Bioactive Glass Composites
Keywords
Sirna, Collagen, Bioactive Glass, Osteogenic
Abstract
Objective To explore the osteogenic effect of siRNA-loaded collagen/bioactive glass composite materials. Methods Three groups of composite materials were prepared by freeze-drying method: collagen/bioglass, negative control siRNA-loaded collagen/bioglass, and noggin-loaded collagen/bioglass. The CCK8 test was used to detect the effect of the extracts of the above three groups of different scaffold materials on cell proliferation in the blank group. ALP activity detection, q-PCR detection, and alizarin red staining were used to evaluate the effects of the three groups of different scaffolds on the mineralization of MC3T3 cells. Results After MC3T3 cells were co-cultured with the material extract for 3 and 5 days, the number of cell proliferation in the collagen/bioglass composite, negative control siRNA-loaded collagen/bioglass composite, and noggin-loaded collagen/bioglass scaffold groups was significantly higher than that in the blank group. , the difference is statistically significant (P<0.05). After 14 days of culture, the ALP activity of the siRNA-loaded collagen/bioglass scaffold group was higher than that of the simple scaffold group, and the difference was statistically significant (P<0.05). After 14 days of culture, the expression levels of ALP, Runx2, and BSP in the siRNA-loaded collagen/bioglass scaffold group were higher than those in the simple scaffold group, and the difference was statistically significant (P<0.05). Alizarin red staining results showed that the siRNA-loaded collagen/bioglass scaffold group had more mineralized nodules than the other groups, and the difference was statistically significant (P<0.05). Conclusion The siRNA-loaded collagen/bioglass composite scaffold has good biocompatibility, and the collagen/bioglass composite and siRNA noggin can synergistically promote osteogenesis. For further information of this article and research, feel free to contact our team for asssitance.
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