Preparation Of Collagen Scaffold Material Composite Membrane And Observation Of Tissue Culture Performance
Keywords
Collagen, Chitose, Hyaluronic Acid
Abstract
Objective To prepare pure collagen membrane, collagen/ha membrane, collagen/chitose membrane and collagen/hyaluronic acid/chitose membrane (referred to as collagen composite membrane), and study their tissue culture properties. Methods The in vitro degradation time and cell proliferation number were measured respectively. Results (1) The degradation time was: 1380 min for pure collagen membrane, 1376 min for collagen/ha membrane, and 1560 min for collagen composite membrane. (2) On collagen/ha membrane and collagen composite membrane, the number of proliferating cells is always the largest. Conclusion (1) Adding chitose is beneficial to reducing the degradation rate of collagen membrane in vitro, but hyaluronic acid does not. (2) Adding hyaluronic acid is beneficial to the adsorption, growth, and proliferation of cells on the collagen membrane, but chitose does not.
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Original research was done by Huang Hanping, Mou Shansong, Ma Ande
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