Preparation Of Yak Collagen Polypeptide By Enzymatic Hydrolysis And Evaluation Of Its Processing Properties
Keywords
Yak Bone Alkaline Protease Peptide Response Surface Analysis Processing Characteristics Yak Bone, Alkaline Protease, Peptides, Response Surface Analysis
Abstract
Using yak bone as raw material, soluble collagen peptides were prepared by enzymatic hydrolysis. The effects of protease type, substrate mass concentration, enzyme base ratio, enzymatic time, pH, and enzymatic temperature on the hydrolysis degree of the enzymatic solution and peptide yield were investigated. Based on the single factor experiment, a response surface optimization experiment was conducted with the peptide yield as an indicator, and alkaline protease was finally determined to be the most suitable enzyme for the experiment. The optimal enzymatic hydrolysis conditions were: substrate mass concentration 3.5 g/dL, enzyme-to-base ratio 4 000 U /g, pH 7.0, enzymatic hydrolysis time 4 h, at this time the peptide yield was 22.06%. The processing characteristics of the enzymatic hydrolyzate showed that it had good solubility and stability. Yak bone protein was hydrolyzed with protease for the preparation of soluble polypeptides. Alkaline protease was proved to be the optimal activator and the single-factor method was used to analyze the effect of time, pH, substrate concentration, enzyme/substrate ratio and temperature on the degree of hydrolysis and yield of polypeptides. Factors such as substrate concentration, enzyme/substrate ratio, hydrolysis pH and time were screened out for response surface analysis based on polypeptides yield. On the optimal hydrolysis conditions of substrate concentration 3.5 g/dL, enzyme /substrate ratio 4 000 U/g, hydrolysis pH 7.0 and time 4 h, the actual value of yield was 22.06% and the peptides were detected with good solubility and stability
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Original research was done by Liu Jing, Li Cheng, Hu Yixiao, Du Xin, Zhou Hengliang, Liu Aiping, Gu Yu, Peng Lulu
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