Research On Constructing Type Iv Collagen/Heparin Biolayer On Ti Surface To Improve Cell Compatibility*
Keywords
Titanium, Electrostatic Interaction, Type Iv Collagen, Heparin, Endothelial Progenitor Cells
Abstract
Endothelialization on the surface of the scaffold has become a good way to ultimately achieve long-term compatibility between the scaffold and blood and tissue. The accumulation, growth and proliferation of endothelial progenitor cells on the surface of the material make rapid endothelialization possible. This article uses electrostatic binding to combine the anticoagulant drug heparin and type IV collagen, an important component of the endothelial cell basement membrane, to the titanium surface, making it have good cell compatibility. After alkali activation and silanization treatment, titanium forms a positively charged amino surface, which electrostatically combines with the heparin/type IV collagen complex with residual negative charges to form a biochemical layer. The changes of characteristic groups on the surface of the material were detected through Fourier transform infrared grazing, and the changes in surface hydrophobicity and surface morphology were tracked through measurement of water contact angle and scanning electron microscope. Qualitative characterization of type IV collagen by immunofluorescence staining. The results showed that the biomimetic layer was successfully constructed. The modified surface was able to adhere to more endothelial progenitor cells than the titanium surface. The cells grew and multiplied faster, the cell morphology was good, and the cell compatibility was significantly improved. The introduction of type IV collagen has important reference value for the surface modification of cardiovascular implantable devices
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Original research was done by Zhang Kun, Li Jing’an, Wang Xue, Deng Kun, Chen Junying, Huang Nan
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