Response Surface Methodology To Optimize The Extraction Process Of Collagen From Horse-Faced Fish Skin And Analysis Of Collagen Composition
Keywords
Horsetail Fish, Fish Skin, Collagen, Extraction, Amino Acid Analysis
Abstract
This article aims to use response surface methodology to optimize the extraction process of collagen from horse-faced fish skin and analyze its amino acid composition, so as to provide a certain theoretical basis for the deep processing and high-value utilization of horse-faced fish skin. The results show that the pre-treatment conditions for collagen extraction are: 10% isopropyl alcohol is used for degreasing, the material-to-liquid ratio is 1:15, and the time is 20 hours; 7.5% NaCl solution is used to remove impurity proteins, the material-to-liquid ratio is 1:10, and the time is 24 hours. The optimal extraction process of collagen is: 0.38mol¡¤L-1 acetic acid solution, solid-liquid ratio 1:30, time 33h. Under these conditions, the actual yield is 362.5mg¡¤g-1. Amino acid analysis showed that the glycine content was 24.12%, and its residues accounted for about 1/3 of the total residues. The hydroxyproline content was 9.31%, and tryptophan was not detected, which is consistent with the characteristics of collagen.
For further information of this article and research, feel free to contact our team for asssitance.
Original research was done by Xia Shanshan, Cao Shaoqian, Liu Liang, Qi Xiangyang
About ETChem
ETChem, a reputable Chinese Collagen factory manufacturer and supplier, is renowned for producing, stocking, exporting, and delivering the highest quality collagens. They include marine collagen, fish collagen, bovine collagen, chicken collagen, type I collagen, type II collagen and type III collagen etc. Their offerings, characterized by a neutral taste, and instant solubility attributes, cater to a diverse range of industries. They serve nutraceutical, pharmaceutical, cosmeceutical, veterinary, as well as food and beverage finished product distributors, traders, and manufacturers across Europe, USA, Canada, Australia, Thailand, Japan, Korea, Brazil, and Chile, among others.
ETChem specialization includes exporting and delivering tailor-made collagen powder and finished collagen nutritional supplements. Their extensive product range covers sectors like Food and Beverage, Sports Nutrition, Weight Management, Dietary Supplements, Health and Wellness Products, ensuring comprehensive solutions to meet all your protein needs.
As a trusted company by leading global food and beverage brands and Fortune 500 companies, ETChem reinforces China’s reputation in the global arena. For more information or to sample their products, please contact them and email karen(at)et-chem.com today.