Response Surface Optimization Of Collagen Peptide Enzymatic Hydrolysis Process And Antioxidant Activity Of Squid Tentacle Peeling Liquid
Keywords
Squid Tentacle Peeling Liquid, Enzymatic Hydrolysis, Antioxidant, Degree Of Hydrolysis, Half Inhibitory Concentration
Abstract
Objective: To study the enzymatic hydrolysis process and antioxidant activity of collagen peptides in Peruvian squid tentacle peeling liquid. Method: Using the degree of hydrolysis and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging rate as indicators, single factor experiment and response surface method were used to analyze The enzymatic hydrolysis process was optimized and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (2,2′-azinobis-(3-ethylbenzothiazoline-6) -sulfonic acid) diammonium salt, ABTS) free radicals and? The OH scavenging rate and ferrous iron reducing capacity were used to evaluate the antioxidant activity of the enzymatic hydrolyzate and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel exclusion chromatography. Its molecular mass distribution. Results: The optimal enzymatic hydrolysis process was as follows: enzymatic hydrolysis temperature 50 ¡æ, initial pH 7.4, substrate protein mass fraction 3.2%, enzymatic hydrolysis time 3.7 h, and enzyme addition amount 3 000 U/g. Under these conditions, the degree of hydrolysis and the DPPH free radical scavenging rate of the enzymatic hydrolysis product were (37.23¡À0.08)% and (43.61¡À0.09)% respectively. For ABTS+? and? The IC50 of OH scavenging rate were 0.37 mg/mL and 0.41 mg/mL respectively, and showed strong ferrous iron reducing ability; SDS-PAGE showed that under the optimal enzymatic hydrolysis process, the protein was basically hydrolyzed into small molecule peptides; gel Exclusion chromatography analysis showed that the molecular mass of the enzymatic hydrolysis product was between 1 and 5 kD. Conclusion: The enzymatic hydrolysis product has strong antioxidant activity and can be fully developed and utilized as a base material for antioxidant active substances.
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Original research was done by Yu Hui, Wu Yangyang, Sun Haiyan, Jiang Xuhua, Luo Chunyan, Chen Xiao’e
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