Study On Extraction Technology Of Collagen From Freshwater Fish Skin (¢ñ)
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Abstract
Abstract The extraction process of collagen from the skin of four freshwater fish species, tilapia, bighead carp, grass carp and crucian carp, was studied. The results showed that the degreasing effect of fish skin with ether twice was significant, and the degreasing rates were 92.4%, 95.1% and 66.8 respectively. % and 76.7%, the extraction rate and yield of collagen after fat removal are slightly increased. Unless the collagen process is: first soaked in NaOH for 6 h, and then soaked in NaCl for 6 h, the corresponding extraction of four kinds of fish skin collagen The yields were 34.4%, 28.7%, 31.5% and 42.5% respectively, and the yields were 8.4%, 8.0%, 8.2% and 6.1% respectively, which were 0.4 to 2.3 times, 0.04 to 1.6 times and 0.1 to 0.9 higher than other treatments. times and 0.07 to 0.5 times. The extraction effect of acetic acid is better than that of citric acid, with corresponding extraction rates of 34.8%, 23.6%, 33.6%, and 43.0% respectively, and the yields are 8.5%, 6.6%, 8.63%, and 6.19%, respectively. Citric acid is 2.4 to 4.3 times higher and 1.7 to 4.0 times higher respectively. Temperature has a greater impact on the extraction of collagen from the skin of tilapia, bighead carp and grass carp. The optimal extraction temperature is 20¡ãC, and the corresponding extraction rates are 34.05% in turn. , 27.05%, 33.38%, and the yields are 8.32%, 7.54%, and 8.58% respectively; temperature has little effect on the extraction of crucian carp skin collagen, and the extraction rate and yield are approximately 42% and 6, respectively, in the range of 520 ¡æ. %about
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